在埃及，对布鲁氏菌病阴性的荷斯坦奶牛群中，用流产布鲁氏菌RB51疫苗免疫后从流产胎儿中分离出的菌株进行分子分型。
Molecular typing of isolates obtained from aborted foetuses in Brucella-free Holstein dairy cattle herd after immunisation with Brucella abortus RB51 vaccine in Egypt.
作者信息
Wareth Gamal, Melzer Falk, Böttcher Denny, El-Diasty Mohamed, El-Beskawy Mohamed, Rasheed Nesma, Schmoock Gernot, Roesler Uwe, Sprague Lisa D, Neubauer Heinrich
机构信息
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany; Faculty of Veterinary Medicine, Benha University, Moshtohor, Toukh 13736, Egypt.
Friedrich-Loeffler-Institut, Federal Research Institute for Animal Health, Institute of Bacterial Infections and Zoonoses, Naumburger Str. 96a, 07743 Jena, Germany.
出版信息
Acta Trop. 2016 Dec;164:267-271. doi: 10.1016/j.actatropica.2016.09.019. Epub 2016 Sep 21.
Bovine brucellosis is endemic in Egypt in spite of application of surveillance and control measures. An increase of abortions was reported in a Holstein dairy cattle herd with 600 animals in Damietta governorate in Egypt after immunisation with Brucella (B.) abortus RB51 vaccine. Twenty one (10.6%) of 197 vaccinated cows aborted after 3 months. All aborted cows had been tested seronegative for brucellosis in the past 3 years. B. abortus was isolated from four foetuses. Conventional biochemical and bacteriological identification and polymerase chain reaction (PCR) confirmed two B. abortus biovar (bv.) 1 smooth and two B. abortus rough strains. None of the B. abortus isolates were identified as RB51. Genotyping analysis by multiple locus of variable number tandem repeats analysis based on 16 markers (MLVA-16) revealed two different profiles with low genetic diversity. B. abortus bv1 was introduced in the herd and caused abortions.
尽管采取了监测和控制措施，但牛布鲁氏菌病在埃及仍呈地方性流行。在埃及达米埃塔省一个拥有600头动物的荷斯坦奶牛群中，用布鲁氏菌（B.）流产RB51疫苗免疫后，报告流产数量增加。197头接种疫苗的奶牛中有21头（10.6%）在3个月后流产。所有流产奶牛在过去3年中布鲁氏菌病血清检测均为阴性。从4个胎儿中分离出流产布鲁氏菌。传统的生化和细菌学鉴定以及聚合酶链反应（PCR）证实有2株光滑型流产布鲁氏菌生物变种（bv.）1和2株粗糙型流产布鲁氏菌菌株。所有流产布鲁氏菌分离株均未鉴定为RB51。基于16个标记的多位点可变数目串联重复序列分析（MLVA-16）进行基因分型分析，结果显示有两种不同的图谱，遗传多样性较低。流产布鲁氏菌bv1传入该牛群并导致流产。
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