简化布鲁氏菌AMOS PCR作为区分流产布鲁氏菌田间菌株分离株与疫苗株19和RB51的快速筛查方法的验证
Validation of the abbreviated Brucella AMOS PCR as a rapid screening method for differentiation of Brucella abortus field strain isolates and the vaccine strains, 19 and RB51.
作者信息
Ewalt D R, Bricker B J
机构信息
National Veterinary Services Laboratories, Veterinary Services, Animal and Plant Health and Inspection Service, U.S. Department of Agriculture, Ames, Iowa 50010, USA.
出版信息
J Clin Microbiol. 2000 Aug;38(8):3085-6. doi: 10.1128/JCM.38.8.3085-3086.2000.
The Brucella AMOS PCR assay was previously developed to identify and differentiate specific Brucella species. In this study, an abbreviated Brucella AMOS PCR test was evaluated to determine its accuracy in differentiating Brucella abortus into three categories: field strains, vaccine strain 19 (S19), and vaccine strain RB51/parent strain 2308 (S2308). Two hundred thirty-one isolates were identified and tested by the conventional biochemical tests and Brucella AMOS PCR. This included 120 isolates identified as B. abortus S19, 9 identified as B. abortus strain RB51, 57 identified as B. abortus biovar 1, 15 identified as B. abortus bv. 2, 1 identified as B. abortus bv. 2 (M antigen dominant), 7 identified as B. abortus bv. 4, and 22 identified as B. abortus S2308 and isolated from experimentally infected cattle. The Brucella AMOS PCR correctly identified each isolate as RB51/S2308, S19, or a field strain of Brucella.
布鲁氏菌AMOS PCR检测方法先前已被开发用于鉴定和区分特定的布鲁氏菌物种。在本研究中，对一种简化的布鲁氏菌AMOS PCR检测进行了评估，以确定其在将流产布鲁氏菌区分为三类的准确性：野外菌株、疫苗株19（S19）和疫苗株RB51/亲本菌株2308（S2308）。通过传统生化检测和布鲁氏菌AMOS PCR对231株分离株进行了鉴定和检测。这包括120株被鉴定为流产布鲁氏菌S19的分离株、9株被鉴定为流产布鲁氏菌RB51菌株、57株被鉴定为流产布鲁氏菌生物变种1、15株被鉴定为流产布鲁氏菌生物变种2、1株被鉴定为流产布鲁氏菌生物变种2（M抗原占主导）、7株被鉴定为流产布鲁氏菌生物变种4以及22株被鉴定为流产布鲁氏菌S2308且从实验感染牛中分离得到的菌株。布鲁氏菌AMOS PCR正确地将每株分离株鉴定为RB51/S2308、S19或布鲁氏菌的野外菌株。
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