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介孔二氧化硅材料在固定化多酚氧化酶中的应用。

Application of mesoporous silica materials for the immobilization of polyphenol oxidase.

作者信息

Corell Escuin Paula, García-Bennett Alfonso, Ros-Lis Jose Vicente, Argüelles Foix Angel, Andrés Ana

机构信息

Instituto de Ingeniería de Alimentos para el Desarrollo, Universitat Politècnica de Valencia, Camino de Vera s/n, Valencia, Spain.

ARC Centre of Excellence for Nanoscale BioPhotonics, Department of Chemistry and Biomolecular Science, Macquarie University, Sydney, New South Wales 2109, Australia.

出版信息

Food Chem. 2017 Feb 15;217:360-363. doi: 10.1016/j.foodchem.2016.08.027. Epub 2016 Aug 10.

DOI:10.1016/j.foodchem.2016.08.027
PMID:27664646
Abstract

The ability of a number of mesoporous silica materials (SBA-15, SBA-3, and MCM-48) to immobilize polyphenol oxidase (PPO) at different pH has been tested. Pore size and volume are the structural characteristics with higher influence on the PPO immobilization. Mesoropous material SBA-15 adsorbs a larger quantity of PPO at pH 4.00 and offers an inhibition of enzymatic activity close the 50% in apple extracts.

摘要

已经测试了多种介孔二氧化硅材料(SBA - 15、SBA - 3和MCM - 48)在不同pH值下固定多酚氧化酶(PPO)的能力。孔径和孔体积是对PPO固定化影响较大的结构特征。介孔材料SBA - 15在pH 4.00时吸附大量的PPO,并在苹果提取物中使酶活性抑制接近50%。

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