不同品种蓝莓（越橘属）的植物化学特征、抗氧化及细胞抗氧化活性比较

Comparison of phytochemical profiles, antioxidant and cellular antioxidant activities of different varieties of blueberry (Vaccinium spp.).

作者信息

Wang Huailing, Guo Xinbo, Hu Xiaodan, Li Tong, Fu Xiong, Liu Rui Hai

机构信息

School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, China.

School of Food Sciences and Engineering, South China University of Technology, Guangzhou 510641, China; Department of Food Science, Stocking Hall, Cornell University, Ithaca, NY 14853, USA.

出版信息

Food Chem. 2017 Feb 15;217:773-781. doi: 10.1016/j.foodchem.2016.09.002. Epub 2016 Sep 3.

DOI:10.1016/j.foodchem.2016.09.002

PMID:27664697

Abstract

Numerous reports have demonstrated that the consumption of fruits and vegetables is beneficial for the human health. Blueberries, in particular, are rich in phytochemicals including free and bound forming. Phytochemical profiles of 14 varieties of blueberry were compared in this study. 12 compounds were analyzed and had significant changes in blueberry fruits. Total antioxidant activities in different blueberry varieties varied about 2.6times by oxygen radical absorbance capacity (ORAC) assay, and 2times by peroxyl radical scavenging capacity (PSC) assay. The cellular antioxidant activities (CAA) in different varieties varied about 3.9times without phosphate buffer saline (PBS) wash, and 4.7times with PBS wash by CAA assay. Blueberry extracts had potent antiproliferative activities against HepG2 human liver cancer cells, indicating the potential protective benefits associated with their use as functional foods. The anti-proliferative activity was observed to be dose-dependent in blueberry extracts.

摘要

众多报告表明，食用水果和蔬菜对人体健康有益。特别是蓝莓，富含包括游离态和结合态在内的植物化学物质。本研究比较了14个蓝莓品种的植物化学特征。分析了12种化合物，其在蓝莓果实中发生了显著变化。通过氧自由基吸收能力（ORAC）测定，不同蓝莓品种的总抗氧化活性相差约2.6倍；通过过氧自由基清除能力（PSC）测定，相差约2倍。通过细胞抗氧化活性（CAA）测定，不同品种在不使用磷酸盐缓冲盐水（PBS）洗涤时，细胞抗氧化活性相差约3.9倍；使用PBS洗涤时，相差约4.7倍。蓝莓提取物对人肝癌细胞HepG2具有强大的抗增殖活性，表明其作为功能性食品具有潜在的保护作用。观察到蓝莓提取物的抗增殖活性呈剂量依赖性。