Yamamoto N, Lopes L C, Campello A P, Klüppel M L
Departamento de Bioquímica Universidade Federal do Paraná, Brasil.
Cell Biochem Funct. 1989 Apr;7(2):129-34. doi: 10.1002/cbf.290070208.
Effects of methotrexate (MTX) on mitochondrial oxidative metabolism and ion transport were studied. MTX decreases the membrane potential (delta psi) upon energization of the mitochondrial membrane by NAD+-linked substrates and decreases the amplitude and velocity of swelling induced by glutamate and alpha-ketoglutarate. MTX also has an inhibitory effect on the activities of the oxidation enzymes of NAD+-linked substrates without interfering with the oxidation systems of FAD-linked substrates. The effects of MTX could be interpreted as a consequence of a decrease in the ionic conductivity of the mitochondrial inner membrane.
