Chen Yu, Ni Wei, Yan Huan, Qin Xu-Jie, Khan Afsar, Liu Hui, Shu Tong, Jin Ling-Yu, Liu Hai-Yang
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; University of Chinese Academy of Sciences, Beijing 100039, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming, 650201, China.
State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences, Kunming 650201, China; Yunnan Key Laboratory of Natural Medicinal Chemistry, Kunming, 650201, China.
Phytochemistry. 2016 Nov;131:165-173. doi: 10.1016/j.phytochem.2016.09.006. Epub 2016 Sep 22.
Ten spirostanol glycosides, trillikamtosides A-J, together with eleven known analogues, were isolated from the hemostatic fraction of the 75% aqueous EtOH extract of the whole herbs of Trillium kamtschaticum. Their structures were established by extensive spectroscopic data analysis and chemical methods. The aglycones of three of these compounds had unique 3β,17α-dihydroxy-spirostanes featuring a double bond between C-4 and C-5, while two others represent a rare class of spirostanol glycosides which possess a 5(6 → 7) abeo-steroidal aglycone. All the compounds were evaluated for their hemostatic and antimicrobial activities. Three of the spirostanol glycosides exhibited induced-platelet aggregation at a concentration of 300 μg/mL with maximal induced-platelet aggregation rates of 72%, 71%, and 62% in rabbits, respectively, and their EC values were 492.7, 203.3, and 109.8 μM. Five of the spirostanol glycosides showed an anti-Candida albicans effect with MIC values of 21.1, 10.6, 8.8, 21.6, and 11.0 μM, respectively.
从东北延龄草全草75%乙醇提取物的止血部位分离得到10个螺甾烷醇苷，即延龄草苷A-J，以及11个已知类似物。通过广泛的光谱数据分析和化学方法确定了它们的结构。其中3个化合物的苷元具有独特的3β,17α - 二羟基螺甾烷结构，C-4和C-5之间有一个双键，另外2个则代表一类罕见的螺甾烷醇苷，其具有5(6→7)降甾类苷元。对所有化合物的止血和抗菌活性进行了评估。3个螺甾烷醇苷在浓度为300μg/mL时可诱导兔血小板聚集，最大诱导血小板聚集率分别为72%、71%和62%，其EC值分别为492.7、203.3和109.8μM。5个螺甾烷醇苷对白色念珠菌有抑制作用，MIC值分别为21.1、10.6、8.8、21.6和11.0μM。
