Tang Rui, Zhang Feng, Kone N'Golopé, Chen Jing-Hua, Zhu Fen, Han Ri-Chou, Lei Chao-Liang, Kenis Marc, Huang Ling-Qiao, Wang Chen-Zhu
MoA-CABI Joint Laboratory for Bio-safety, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, 2 Yuanmingyuan West Road, Beijing 100193, P. R. China.
State Key Laboratory of Integrated Management of Pest Insects and Rodents, Institute of Zoology, Chinese Academy of Sciences, 1-5 Beichen West Road, Beijing 100101, P. R. China.
Sci Rep. 2016 Sep 26;6:33017. doi: 10.1038/srep33017.
Oviposition attractants for the house fly Musca domestica have been investigated using electrophysiological tests, behavioural assays and field tests. Volatiles were collected via head space absorption method from fermented wheat bran, fresh wheat bran, rearing substrate residue and house fly maggots. A Y-tube olfactometer assay showed that the odor of fermented wheat bran was a significant attractant for female house flies. Bioactive compounds from fermented wheat bran for house fly females were identified by electrophysiology and mass spectrophotometry and confirmed with standard chemicals. Four electrophysiologically active compounds including ethyl palmitate, ethyl linoleate, methyl linoleate, and linoleic acid were found at a proportion of 10:24:6:0.2. Functional imaging in the female antennal lobes revealed an overlapped active pattern for all chemicals. Further multiple-choice behavioural bioassays showed that these chemicals, as well as a mixture that mimicked the naturally occurring combination, increased the attractiveness of non-preferred rearing substrates of cotton and maize powder. Finally, a field demonstration test revealed that, by adding this mimic blend into a rearing substrate used to attract and breed house flies in West Africa, egg numbers laid by females were increased. These chemicals could be utilized to improve house fly production systems or considered for lure traps.
已通过电生理测试、行为测定和田间试验对家蝇（Musca domestica）的产卵引诱剂进行了研究。通过顶空吸附法从发酵麦麸、新鲜麦麸、饲养基质残渣和家蝇蛆中收集挥发物。Y型管嗅觉测定试验表明，发酵麦麸的气味对雌性家蝇具有显著的引诱作用。通过电生理学和质谱法鉴定了发酵麦麸中对雌性家蝇具有生物活性的化合物，并用标准化学物质进行了确认。发现四种具有电生理活性的化合物，即棕榈酸乙酯、亚油酸乙酯、亚油酸甲酯和亚油酸，其比例为10:24:6:0.2。对雌性触角叶的功能成像显示，所有化学物质都有重叠的活性模式。进一步的多项选择行为生物测定表明，这些化学物质以及模拟天然组合的混合物，增加了棉花和玉米粉等非首选饲养基质的吸引力。最后，一项田间示范试验表明，在西非用于吸引和饲养家蝇的饲养基质中添加这种模拟混合物后，雌性家蝇的产卵量增加了。这些化学物质可用于改进家蝇生产系统或考虑用于诱捕器。
