Zhao Yao, Wang Wanqiang, Zhu Fen, Wang Xiaoyun, Wang Xiaoping, Lei Chaoliang
Hubei International Cooperation Base for Waste Conversion by Insects, Huazhong Agricultural University, No. 1 Shizishan Street, Hongshan District, Wuhan, 430070, China.
Hubei Insect Resources Utilization and Sustainable Pest Management Key Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.
AMB Express. 2017 Dec;7(1):147. doi: 10.1186/s13568-017-0445-7. Epub 2017 Jul 10.
House fly larvae provide a prolific and sustainable source of proteins used in poultry and fish feed. Wheat bran is a superior diet for house fly larvae and has been widely investigated to exploit its potential in the food and feed area. Using Illumina MiSeq 16S rDNA sequencing, this study investigated the gut microbiota of house fly larvae feeding on wheat bran and the bacterial community in the wheat bran. The bacterial communities in the house fly larvae were dominated by the phyla Proteobacteria and Firmicutes. Enterobacteriaceae and Providencia were the predominant bacteria at the family and genus levels, respectively. Some bacteria in the phyla Actinobacteria, Proteobacteria, Bacteroidetes and Firmicutes may be transferred from the gut of house flies to the wheat bran during feeding and may be involved in degrading and utilizing polysaccharides in the cell wall of wheat bran. The significance of the gut microbiota of house fly larvae, their transferring and roles in degradation of wheat bran is discussed. These findings regarding the gut microbiota of house fly larvae will provide opportunities for research on the impact of microbial communities on poultry and fish.
家蝇幼虫为家禽和鱼类饲料提供了丰富且可持续的蛋白质来源。麦麸是家蝇幼虫的优质食物，人们已对其在食品和饲料领域的潜力展开了广泛研究。本研究利用Illumina MiSeq 16S rDNA测序技术，调查了以麦麸为食的家蝇幼虫的肠道微生物群以及麦麸中的细菌群落。家蝇幼虫体内的细菌群落以变形菌门和厚壁菌门为主。肠杆菌科和普罗威登斯菌属分别是在科和属水平上的主要细菌。放线菌门、变形菌门、拟杆菌门和厚壁菌门中的一些细菌可能在进食过程中从家蝇肠道转移至麦麸，并且可能参与麦麸细胞壁中多糖的降解和利用。本文讨论了家蝇幼虫肠道微生物群的重要性、它们的转移以及在麦麸降解中的作用。这些关于家蝇幼虫肠道微生物群的研究结果将为研究微生物群落对家禽和鱼类的影响提供契机。
