Wang Chengyan, Zhao Kai, Zhang Rui, Jiang Li, Wang Rui, Zhang Xiaodong, Wang He, Jin Lixin, Zhang Jue, Wang Xiaoying, Fang Jing
Academy for Advanced Interdisciplinary Studies, Peking University, Beijing, China.
Department of Radiology, Peking University First Hospital, Beijing, China.
NMR Biomed. 2016 Nov;29(11):1601-1607. doi: 10.1002/nbm.3625. Epub 2016 Sep 27.
The purpose of this study is to demonstrate the feasibility of dynamic renal R /R '/R * measurements based on a method, denoted psMASE-ME, in which a periodic 180° pulse-shifting multi-echo asymmetric spin echo (psMASE) sequence, combined with a moving estimation (ME) strategy, is adopted. Following approval by the institutional animal care and use committee, a block design of respiratory challenge with interleaved air and carbogen (97% O , 3% CO ) breathing was employed in nine rabbits. Parametrical R /R '/R * maps were computed and average R /R '/R * values were measured in regions of interest in the renal medulla and cortex. Bland-Altman plots showed good agreement between the proposed method and reference standards of multi-echo spin echo and multi-echo gradient echo sequences. Renal R , R ' and R * decreased significantly from 16.2 ± 4.4 s , 9.8 ± 5.2 s and 25.9 ± 5.0 s to 14.9 ± 4.4 s (p < 0.05), 8.5 ± 4.1 s (p < 0.05) and 23.4 ± 4.8 s (p < 0.05) in the cortex when switching the gas mixture from room air to carbogen. In the renal medulla, R , R ' and R * also decreased significantly from 12.9 ± 4.7 s , 15.1 ± 5.8 s and 27.9 ± 5.3 s to 11.8 ± 4.5 s (p < 0.05), 14.2 ± 4.2 s (p < 0.05) and 25.8 ± 5.1 s (p < 0.05). No statistically significant differences in relative R , R ' and R * changes were observed between the cortex and medulla (p = 0.72 for R , p = 0.39 for R ' and p = 0.61 for R *). The psMASE-ME method for dynamic renal R /R '/R * measurements, together with the respiratory challenge, has potential use in the evaluation of renal oxygenation in many renal diseases.
本研究的目的是基于一种称为psMASE-ME的方法来证明动态肾R/R'/R测量的可行性，该方法采用了周期性180°脉冲移位多回波不对称自旋回波（psMASE）序列，并结合了运动估计（ME）策略。经机构动物护理和使用委员会批准，对9只兔子采用了空气和卡波金（97%O₂，3%CO₂）呼吸交替的呼吸刺激块设计。计算了参数化的R/R'/R图，并在肾髓质和皮质的感兴趣区域测量了平均R/R'/R值。Bland-Altman图显示，所提出的方法与多回波自旋回波和多回波梯度回波序列的参考标准之间具有良好的一致性。当气体混合物从室内空气切换到卡波金时，皮质中的肾R、R'和R显著降低，从16.2±4.4 s、9.8±5.2 s和25.9±5.0 s降至14.9±4.4 s（p<0.05）、8.5±4.1 s（p<0.05）和23.4±4.8 s（p<0.05）。在肾髓质中，R、R'和R也显著降低，从12.9±4.7 s、15.1±5.8 s和27.9±5.3 s降至11.8±4.5 s（p<0.05）、14.2±4.2 s（p<0.05）和25.8±5.1 s（p<0.05）。皮质和髓质之间在相对R、R'和R变化方面未观察到统计学上的显著差异（R为p=0.72，R'为p=0.39，R为p=0.61）。用于动态肾R/R'/R测量的psMASE-ME方法，连同呼吸刺激，在许多肾脏疾病的肾氧合评估中具有潜在用途。
