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位于人类雌激素受体激素结合域的转录激活功能并非由单个外显子编码。

The transcriptional activation function located in the hormone-binding domain of the human oestrogen receptor is not encoded in a single exon.

作者信息

Webster N J, Green S, Tasset D, Ponglikitmongkol M, Chambon P

机构信息

Unité 184 de Biologie Moléculaire et de Génie Génétique de l'INSERM, Faculté de Médecine, Strasbourg, France.

出版信息

EMBO J. 1989 May;8(5):1441-6. doi: 10.1002/j.1460-2075.1989.tb03526.x.

DOI:10.1002/j.1460-2075.1989.tb03526.x
PMID:2767048
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC400972/
Abstract

Using GAL4 chimeric receptors, we have reported previously that the hormone-binding domain (HBD) of the human oestrogen receptor (hER) contains an hormone-inducible transcription activation function. We have extended that study here to show that this activation function represents the major activating domain in the hER in HeLa cells. In addition, we have expressed the various exons encoding the hER HBD as GAL4 fusion proteins and have shown that none contain a discrete activation function. Thus the activating domain of the hER HBD appears to be different from the recently characterized 'simple' activating domains, such as acidic 'blob' or amphipathic helix, and more likely corresponds to a protein surface created from dispersed elements and dependent upon the three-dimensional folding of the HBD.

摘要

利用GAL4嵌合受体，我们先前已报道人雌激素受体（hER）的激素结合结构域（HBD）含有激素诱导的转录激活功能。我们在此扩展了该研究，以表明这种激活功能代表了HeLa细胞中hER的主要激活结构域。此外，我们将编码hER HBD的各个外显子表达为GAL4融合蛋白，并表明它们均不包含离散的激活功能。因此，hER HBD的激活结构域似乎与最近表征的“简单”激活结构域不同，如酸性“斑点”或两亲性螺旋，更可能对应于由分散元件形成并依赖于HBD三维折叠的蛋白质表面。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5917/400972/278ecdfce764/emboj00129-0155-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5917/400972/3d7ee321991c/emboj00129-0153-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5917/400972/0748eeb9c387/emboj00129-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5917/400972/15866fdafb6e/emboj00129-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5917/400972/278ecdfce764/emboj00129-0155-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5917/400972/3d7ee321991c/emboj00129-0153-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5917/400972/0748eeb9c387/emboj00129-0154-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5917/400972/15866fdafb6e/emboj00129-0155-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5917/400972/278ecdfce764/emboj00129-0155-b.jpg

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