Berlingieri M T, Musti A M, Avvedimento V E, Di Lauro R, Di Fiore P P, Fusco A
Dipartimento di Biologia e Patologia Cellulare e Molecolare e/o Centro di Endocrinologia ed Oncologia Sperimentale del C.N.R., Università di Napoli, Italy.
Exp Cell Res. 1989 Aug;183(2):277-83. doi: 10.1016/0014-4827(89)90388-1.
Transformation of rat thyroid epithelial cells by Kirsten murine sarcoma virus results in the block of certain thyroid differentiated functions, such as synthesis and secretion of thyroglobulin. Our studies, performed by a run-on assay, demonstrate that this block occurs at the transcriptional level. We also demonstrate the de novo methylation of two methylation-sensitive sites, located within the 5' end regulatory sequences of the thyroglobulin gene, in transformed cells, in the absence of any rearrangement of the gene. These two methylation-sensitive sites were methylated also in a rat thyroid cell line transformed by another retrovirus and in two normal cell lines which do not express the thyroglobulin gene.
克尔斯滕鼠肉瘤病毒使大鼠甲状腺上皮细胞发生转化，导致某些甲状腺分化功能受阻，如甲状腺球蛋白的合成和分泌。我们通过连续转录分析进行的研究表明，这种阻断发生在转录水平。我们还证明，在转化细胞中，甲状腺球蛋白基因5'端调控序列内的两个甲基化敏感位点发生了从头甲基化，而该基因没有任何重排。在另一种逆转录病毒转化的大鼠甲状腺细胞系以及两个不表达甲状腺球蛋白基因的正常细胞系中，这两个甲基化敏感位点也发生了甲基化。
