Ferrentino M, Di Fiore P P, Fusco A, Colletta G, Pinto A, Vecchio G
J Gen Virol. 1984 Nov;65 ( Pt 11):1955-61. doi: 10.1099/0022-1317-65-11-1955.
A systematic study has been performed using a series of differentiated rat thyroid epithelial cell lines either uninfected or infected with Kirsten murine sarcoma virus (KiMSV), to determine the levels of the p21 product of the v-ras-Ki oncogene in transformed and normal cell lines. The p21 levels have been assayed by SDS-polyacrylamide gel electrophoresis of immunoprecipitates of 35S-labelled cell extracts and by a GDP binding assay. All cell lines analysed showed a significant increase in the levels of p21 after transformation with KiMSV compared to the p21 levels of uninfected and untransformed differentiated thyroid cells. The results reported here confirm the potential ability of the v-ras-Ki oncogene product to transform epithelial cells. They show, furthermore, that not only is p21 present in some epithelial cells transformed by KiMSV, but also that it is functionally active, as has been shown for fibroblasts transformed by the same virus, and that its functioning is maintained after passaging in vivo of the transformed cells.
利用一系列未感染或感染了 Kirsten 小鼠肉瘤病毒（KiMSV）的分化大鼠甲状腺上皮细胞系进行了一项系统性研究，以确定 v-ras-Ki 癌基因的 p21 产物在转化细胞系和正常细胞系中的水平。通过对 35S 标记的细胞提取物免疫沉淀物进行 SDS-聚丙烯酰胺凝胶电泳以及 GDP 结合试验来测定 p21 水平。与未感染和未转化的分化甲状腺细胞的 p21 水平相比，所有分析的细胞系在用 KiMSV 转化后 p21 水平均显著增加。此处报道的结果证实了 v-ras-Ki 癌基因产物转化上皮细胞的潜在能力。此外，结果表明，p21 不仅存在于一些被 KiMSV 转化的上皮细胞中，而且具有功能活性，这与被同一病毒转化的成纤维细胞的情况相同，并且在转化细胞体内传代后其功能仍得以维持。
