Liu Fang, He Yuan, Cao Qinghua, Liu Ni, Zhang Wenhui
Department of Oncology, Nanfang Hospital, Southern Medical University, Guangzhou 510515, China.
State Key Laboratory of Oncology in South China, Collaborative Innovation Center for Cancer Medicine and Department of Molecular Diagnostics, Sun Yat-sen University Cancer Center, Guangzhou 510060, China.
Dis Markers. 2016;2016:2436518. doi: 10.1155/2016/2436518. Epub 2016 Sep 8.
. To investigate the expression of transducin- (-) like 1 X-linked receptor 1 (TBL1XR1) in human gastric cancer (GC) and its correlation with prognostic and biologic significance. . TBL1XR1 mRNA expression was analyzed in gastric cancer using a microarray dataset (GSE2701) from the Gene Expression Omnibus (GEO). Immunohistochemistry (IHC) analysis of TBL1XR1 was performed on GC tissue microarray (TMA) to assess its prognostic and biological significance in 334 patients of GC. . Analysis of GSE2701 showed that the mRNA levels of TBL1XR1 were significantly elevated in primary gastric tumor and lymph node tissues than normal gastric tissues ( < 0.05). The same results of TBL1XR1 protein level were observed by IHC staining in 334 GC tissues. 204 of 334 (60.1%) primary gastric cancer tissues showed high expression of TBL1XR1 protein. TBL1XR1 overexpression was significantly correlated with lymph node metastasis ( = 0.000) and advanced TNM stage ( = 0.001). Moreover, high levels of TBL1XR1 predicted worse overall survival ( = 0.015). Multivariate Cox regression analysis indicated that high expression of TBL1XR1 was an independent prognostic factor for poor overall survival (HR, 0.525; 95% confidence interval, 0.367-0.752; = 0.005). . This present study demonstrates that TBL1XR1 is overexpressed in gastric cancer and may be a potential predictor and therapeutic target for GC patients.
. 探讨转导素样 1 X 连锁受体 1（TBL1XR1）在人胃癌（GC）中的表达及其与预后的相关性和生物学意义。. 使用来自基因表达综合数据库（GEO）的微阵列数据集（GSE2701）分析胃癌中 TBL1XR1 mRNA 的表达。对 334 例胃癌患者的胃组织芯片（TMA）进行 TBL1XR1 的免疫组织化学（IHC）分析，以评估其预后和生物学意义。. 对 GSE2701 的分析表明，原发性胃癌组织和淋巴结组织中 TBL1XR1 的 mRNA 水平显著高于正常胃组织（<0.05）。在 334 例 GC 组织中通过 IHC 染色观察到 TBL1XR1 蛋白水平的相同结果。334 例原发性胃癌组织中有 204 例（60.1%）显示 TBL1XR1 蛋白高表达。TBL1XR1 过表达与淋巴结转移（=0.000）和晚期 TNM 分期（=0.001）显著相关。此外，高水平的 TBL1XR1 预示总体生存率较差（=0.015）。多因素 Cox 回归分析表明，TBL1XR1 高表达是总体生存不良的独立预后因素（HR，0.525；95%置信区间，0.367 - 0.752；=0.005）。. 本研究表明，TBL1XR1 在胃癌中过表达，可能是 GC 患者的潜在预测指标和治疗靶点。
