Liu Debao, Zhang Yueying, Li Yan, Fan Kaixi
Department of Oncology, Affiliated Hospital of Shandong Academy of Medical Sciences, Jinan, Shandong, China.
School of Medicine and Life Sciences, University of Jinan-Shandong Academy of Medical Sciences, Jinan, Shandong, China.
Oncotarget. 2017 Aug 3;8(47):82910-82919. doi: 10.18632/oncotarget.19876. eCollection 2017 Oct 10.
We investigated whether the () gene was of prognostic relevance to gastric cancer (GC) patients. Immunohistochemical staining of 160 matched GC tumor and adjacent normal tissue samples showed that 58.1% (93/160) of GC samples were NF1-positive as compared to 94.4% (151/160) of normal tissue samples (χ=58.05, <0.001). qRT-PCR analysis revealed that NF1 mRNA expression is lower in GC tissues than normal tissues (χ=34.23, <0.001). Moreover, NF1 protein and mRNA levels were associated with T stage ( <0.05) and TNM ( <0.001). No association was observed with other clinicopathological parameters, including gender, age, tumor size, lymph-node metastasis, cancer differentiation and distant metastasis (all >0.05). Kaplan-Meier analysis revealed that negative or low NF1 were associated with poor overall survival (OS) in gastric cancer patients (<0.001). Further univariate and multivariate cox regression analysis also showed that NF1 expression was an independent risk factor of survival of GC patients. These data show that NF1 has prognostic relevance to clinical outcomes in gastric cancer patients.
我们研究了（）基因是否与胃癌（GC）患者的预后相关。对160对匹配的GC肿瘤组织和癌旁正常组织样本进行免疫组织化学染色，结果显示，93/160（58.1%）的GC样本为NF1阳性，而正常组织样本中这一比例为151/160（94.4%）（χ=58.05，<0.001）。qRT-PCR分析显示，GC组织中NF1 mRNA表达低于正常组织（χ=34.23，<0.001）。此外，NF1蛋白和mRNA水平与T分期（<0.05）和TNM分期（<0.001）相关。未观察到与其他临床病理参数的相关性，包括性别、年龄、肿瘤大小、淋巴结转移、癌症分化和远处转移（均>0.05）。Kaplan-Meier分析显示，NF1阴性或低表达与胃癌患者的总生存期（OS）较差相关（<0.001）。进一步的单因素和多因素cox回归分析也表明，NF1表达是GC患者生存的独立危险因素。这些数据表明，NF1与胃癌患者的临床结局预后相关。
