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载季铵化壳聚糖的二氧化钛纳米管的成骨细胞细胞相容性和增强体内抗感染潜力。

Cytocompatibility with osteogenic cells and enhanced in vivo anti-infection potential of quaternized chitosan-loaded titania nanotubes.

机构信息

Shanghai Key Laboratory of Orthopedic Implants, Department of Orthopedic Surgery, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University, School of Medicine , Shanghai, People's Republic of China.

出版信息

Bone Res. 2016 Sep 20;4:16027. doi: 10.1038/boneres.2016.27. eCollection 2016.

DOI:10.1038/boneres.2016.27
PMID:27672479
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5028847/
Abstract

Infection is one of the major causes of failure of orthopedic implants. Our previous study demonstrated that nanotube modification of the implant surface, together with nanotubes loaded with quaternized chitosan (hydroxypropyltrimethyl ammonium chloride chitosan, HACC), could effectively inhibit bacterial adherence and biofilm formation in vitro. Therefore, the aim of this study was to further investigate the in vitro cytocompatibility with osteogenic cells and the in vivo anti-infection activity of titanium implants with HACC-loaded nanotubes (NT-H). The titanium implant (Ti), nanotubes without polymer loading (NT), and nanotubes loaded with chitosan (NT-C) were fabricated and served as controls. Firstly, we evaluated the cytocompatibility of these specimens with human bone marrow-derived mesenchymal stem cells in vitro. The observation of cell attachment, proliferation, spreading, and viability in vitro showed that NT-H has improved osteogenic activity compared with Ti and NT-C. A prophylaxis rat model with implantation in the femoral medullary cavity and inoculation with methicillin-resistant Staphylococcus aureus was established and evaluated by radiographical, microbiological, and histopathological assessments. Our in vivo study demonstrated that NT-H coatings exhibited significant anti-infection capability compared with the Ti and NT-C groups. In conclusion, HACC-loaded nanotubes fabricated on a titanium substrate show good compatibility with osteogenic cells and enhanced anti-infection ability in vivo, providing a good foundation for clinical application to combat orthopedic implant-associated infections.

摘要

感染是骨科植入物失败的主要原因之一。我们之前的研究表明，通过在植入物表面进行纳米管修饰，并在纳米管中装载季铵化壳聚糖（羟丙基三甲基氯化铵壳聚糖，HACC），可以有效抑制体外细菌黏附和生物膜形成。因此，本研究旨在进一步研究载 HACC 纳米管（NT-H）的钛植入物与成骨细胞的体外细胞相容性和体内抗感染活性。制备了钛植入物（Ti）、无聚合物负载的纳米管（NT）和负载壳聚糖的纳米管（NT-C）作为对照。首先，我们评估了这些标本与骨髓间充质干细胞在体外的细胞相容性。体外观察细胞黏附、增殖、扩散和活力的结果表明，NT-H 与 Ti 和 NT-C 相比具有更好的成骨活性。通过股骨骨髓腔植入和接种耐甲氧西林金黄色葡萄球菌建立了预防大鼠模型，并通过影像学、微生物学和组织病理学评估进行了评价。我们的体内研究表明，NT-H 涂层与 Ti 和 NT-C 组相比具有显著的抗感染能力。总之，载 HACC 的纳米管在钛基底上表现出良好的成骨细胞相容性和增强的体内抗感染能力，为临床应用提供了良好的基础，以对抗骨科植入物相关感染。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/11bc01d1a425/boneres201627-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/c394dd3483ad/boneres201627-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/15e933f44090/boneres201627-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/e4b2482ca158/boneres201627-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/f57b0c5dff34/boneres201627-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/ae37958b4f15/boneres201627-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/f7327864eb3a/boneres201627-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/3ed5b0ba4805/boneres201627-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/11bc01d1a425/boneres201627-f8.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/c394dd3483ad/boneres201627-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/15e933f44090/boneres201627-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/e4b2482ca158/boneres201627-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/f57b0c5dff34/boneres201627-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/ae37958b4f15/boneres201627-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/f7327864eb3a/boneres201627-f6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/3ed5b0ba4805/boneres201627-f7.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6f4c/5028847/11bc01d1a425/boneres201627-f8.jpg

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