Toth G P, Zenick H, Smith M K
Reproductive and Developmental Biochemistry Branch, U.S. Environmental Protection Agency, Cincinnati, Ohio 45268.
Fundam Appl Toxicol. 1989 Jul;13(1):16-25. doi: 10.1016/0272-0590(89)90303-5.
Male and female Long-Evans rats were treated with epichlorohydrin (ECH) by oral gavage (males: 12.5, 25, and 50 mg/kg/day; females: 25, 50, and 100 mg/kg/day) for 21 and 14 days, respectively, prior to mating trials with untreated animals. Treated females were further dosed until delivery. Fertility was assayed in the high-dose males only and was found to be totally impaired. No measured parameters of female reproduction were changed relative to controls. Treated males showed normal copulatory behavior. Sperm morphology and percentage motile sperm were not statistically different from control values in both ejaculated and cauda epididymal samples from ECH-treated animals. The number of sperm in ejaculates was normal while cauda epididymal sperm count was slightly decreased in males at the 50 mg ECH/kg dose level. Mean curvilinear velocity, straight-line velocity, and amplitude of lateral head displacement of cauda epididymal sperm were significantly reduced by ECH at 12.5 mg/kg/day and above. Sperm track linearity was also reduced, but only at 50 mg/kg/day. Beat/cross frequency of sperm was significantly increased at 12.5 mg/kg/day and above. All of the above sperm motion parameters showed dose-dependent trends. These effects are consistent with the spermatozoal metabolic lesions reported for alpha-chlorohydrin, a metabolite of ECH.
在与未处理的动物进行交配试验之前，分别对雄性和雌性Long-Evans大鼠经口灌胃给予环氧氯丙烷（ECH）21天和14天（雄性：12.5、25和50毫克/千克/天；雌性：25、50和100毫克/千克/天）。对处理过的雌性大鼠继续给药直至分娩。仅对高剂量雄性大鼠的生育力进行了测定，发现其完全受损。与对照组相比，未观察到雌性生殖的测量参数有变化。处理过的雄性大鼠表现出正常的交配行为。在来自ECH处理动物的射精样本和附睾尾部样本中，精子形态和活动精子百分比与对照值相比无统计学差异。射精中的精子数量正常，而在50毫克ECH/千克剂量水平的雄性大鼠中，附睾尾部精子计数略有下降。在12.5毫克/千克/天及以上剂量时，ECH可显著降低附睾尾部精子的平均曲线速度、直线速度和侧头位移幅度。精子轨迹线性也降低，但仅在50毫克/千克/天剂量时出现。在12.5毫克/千克/天及以上剂量时，精子的拍击/交叉频率显著增加。上述所有精子运动参数均呈现剂量依赖性趋势。这些影响与ECH的代谢产物α-氯醇所报道的精子代谢损伤一致。
