Komatsu N, Kido A, Kimura Y, Oya M
Department of Legal Medicine, Yamanashi Medical University, Japan.
Forensic Sci Int. 1989 Apr-May;41(1-2):3-9. doi: 10.1016/0379-0738(89)90229-6.
A new reliable and reproducible technique for the simultaneous determination of C6 and C7 types is presented, which employs double replica electroblotting after isoelectric focusing. It permitted clear discrimination of both C6 and C7 components, and the patterns were nearly comparable to those demonstrated separately. The population data obtained by this new technique fitted the genetic hypothesis. The present double replica electroblotting method was successfully applied to the combined phenotyping of C6 and C7 from bloodstains which were stored at room temperature for up to 4 weeks. The method is quite suitable for medicolegal examination of bloodstains particularly for the saving in the amount of sample.
本文介绍了一种同时测定C6和C7类型的新的可靠且可重复的技术，该技术在等电聚焦后采用双重复制品电印迹法。它能够清晰地区分C6和C7成分，其图谱与分别展示的图谱几乎相当。通过这种新技术获得的群体数据符合遗传假设。目前的双重复制品电印迹法已成功应用于对在室温下保存长达4周的血迹中C6和C7的联合表型分析。该方法非常适合血迹的法医检验，特别是在节省样本量方面。
