Song Wei, Liu Li, Lu Hongzhou
Department of Infectious Disease, Shanghai Public Health Clinical Center, Shanghai 201508, China.
Department of Infectious Disease, Shanghai Public Health Clinical Center, Shanghai 201508, China;Department of Infectious Disease, Huashan Hospital, Fudan University, Shanghai 200040, China.
Zhejiang Da Xue Xue Bao Yi Xue Ban. 2016 May 25;45(3):243-248. doi: 10.3785/j.issn.1008-9292.2016.05.04.
To identify isolated from AIDS patients and to evaluate the diagnostic value of MBP64 antigen Colloidal Gold Diagnostic Kit in distinguishing (MTB) and (NTM). A total of 140 isolates from AIDS patients were collected and identified by using 16SrDNA PCR assay as well as immune colloidal gold technique (MBP64 antigen Colloidal Gold Diagnostic Kit). The sensitivity and specificity of Colloidal Gold method was analyzed. With the results of 16SrDNA PCR assay as gold standard, the sensitivity and specificity of Colloidal Gold method in distinguishing MTB and NTM were 96.00% and 96.84%, respectively. Among 140 samples, 114 were identified by 16SrDNA PCR assay, among which 50 (43.86%) were identified as MTB, and 63 (55.26%) were identified as NTM. The most popular NTM in AIDS patients were and . NTM is of high prevalence in AIDS patients, and MBP64 antigen Colloidal Gold Diagnostic Kit can be used as an effective technique in distinguishing NTM and MTB.
从艾滋病患者中分离菌株，并评估MBP64抗原胶体金诊断试剂盒在区分结核分枝杆菌（MTB）和非结核分枝杆菌（NTM）方面的诊断价值。共收集140株艾滋病患者分离菌株，采用16SrDNA PCR检测及免疫胶体金技术（MBP64抗原胶体金诊断试剂盒）进行鉴定，并分析胶体金法的敏感性和特异性。以16SrDNA PCR检测结果为金标准，胶体金法区分MTB和NTM的敏感性和特异性分别为96.00%和96.84%。140份样本中，16SrDNA PCR检测鉴定出114份，其中50份（43.86%）鉴定为MTB，63份（55.26%）鉴定为NTM。艾滋病患者中最常见的NTM是[具体菌名1]和[具体菌名2]。NTM在艾滋病患者中患病率较高，MBP64抗原胶体金诊断试剂盒可作为区分NTM和MTB的有效技术。
