Zhang Wenyao, Li Xuezhong, Xu Tong, Ma Mengru, Zhang Yong, Gao Ming-Qing
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China.
College of Veterinary Medicine, Northwest A&F University, Yangling 712100, Shaanxi, China; Key Laboratory of Animal Biotechnology, Ministry of Agriculture, Northwest A&F University, Yangling 712100, Shaanxi, China.
Exp Cell Res. 2016 Nov 15;349(1):45-52. doi: 10.1016/j.yexcr.2016.09.016. Epub 2016 Sep 25.
Hypernomic secretion of epithelial cytokines has several effects on stromal cells. The contributions of inflammatory epithelial cells to stromal fibroblasts in bovine mammary glands with mastitis remain poorly understood. Here, we established an inflammatory epithelial cell model of bovine mastitis with gram-negative lipopolysaccharide (LPS) and gram-positive lipoteichoic acid (LTA) bacterial cell wall components. We characterized immune responses of mammary stromal fibroblasts induced by inflammatory epithelial cells. Our results showed that inflammatory epithelial cells affected stromal fibroblast characteristics by increasing inflammatory mediator expression, elevating extracellular matrix protein deposition, decreasing proliferation capacity, and enhancing migration ability. The changes in stromal fibroblast proliferation and migration abilities were mediated by signal molecules, such as WNT signal pathway components. LPS- and LTA-induced inflammatory epithelial cells triggered different immune responses in stromal fibroblasts. Thus, in mastitis, bovine mammary gland stromal fibroblasts were affected by inflammatory epithelial cells and displayed inflammation-specific changes, suggesting that fibroblasts play crucial roles in bovine mastitis.
上皮细胞因子的高分泌对基质细胞有多种影响。在患有乳腺炎的牛乳腺中，炎性上皮细胞对基质成纤维细胞的作用仍知之甚少。在此，我们用革兰氏阴性脂多糖（LPS）和革兰氏阳性脂磷壁酸（LTA）细菌细胞壁成分建立了牛乳腺炎炎性上皮细胞模型。我们对炎性上皮细胞诱导的乳腺基质成纤维细胞的免疫反应进行了表征。我们的结果表明，炎性上皮细胞通过增加炎性介质表达、提高细胞外基质蛋白沉积、降低增殖能力和增强迁移能力来影响基质成纤维细胞特性。基质成纤维细胞增殖和迁移能力的变化由信号分子介导，如WNT信号通路成分。LPS和LTA诱导的炎性上皮细胞在基质成纤维细胞中引发了不同的免疫反应。因此，在乳腺炎中，牛乳腺基质成纤维细胞受到炎性上皮细胞的影响并表现出炎症特异性变化，这表明成纤维细胞在牛乳腺炎中起关键作用。
