Li Xuezhong, Wang Hao, Zhang Yanfen, Zhang Jinjing, Qi Shaopei, Zhang Yong, Gao Ming-Qing
College of Veterinary Medicine, Northwest A&F University, Yangling, China.
Northwest A&F University hospital, Northwest A&F University, Yangling, China.
PeerJ. 2019 Apr 5;7:e6715. doi: 10.7717/peerj.6715. eCollection 2019.
The function of long non-coding RNA H19 (H19) on cell proliferation has been observed in various cell types, and the increased expression of H19 was also found in the lipopolysaccharide (LPS)-induced inflammatory bovine mammary epithelial cells (MAC-T). However, the roles of H19 in the inflammatory response and physiological functions of bovine mammary epithelial cell are not clear. In the present study, we found that overexpression of H19 in MAC-T cells significantly promoted cell proliferation, increased the protein and mRNA level of β-casein, and enhanced the expression of tight junction (TJ)-related proteins while inhibited adhesion to cells. In addition, results demonstrated that overexpression of H19 affected the LPS-induced immune response of MAC-T cells by promoting expressions of inflammatory factors, including TNF-α, IL-6, CXCL2 and CCL5, and activating the NF-κB signal pathway. Our findings indicate that H19 is likely to play an important role in maintaining normal functions and regulating immune response of bovine mammary epithelial cells.
长链非编码RNA H19（H19）在多种细胞类型中对细胞增殖的作用已被观察到，并且在脂多糖（LPS）诱导的炎性牛乳腺上皮细胞（MAC-T）中也发现H19的表达增加。然而，H19在牛乳腺上皮细胞的炎症反应和生理功能中的作用尚不清楚。在本研究中，我们发现MAC-T细胞中H19的过表达显著促进细胞增殖，增加β-酪蛋白的蛋白质和mRNA水平，并增强紧密连接（TJ）相关蛋白的表达，同时抑制细胞黏附。此外，结果表明，H19的过表达通过促进包括TNF-α、IL-6、CXCL2和CCL5在内的炎性因子表达并激活NF-κB信号通路，影响LPS诱导的MAC-T细胞免疫反应。我们的研究结果表明，H19可能在维持牛乳腺上皮细胞的正常功能和调节免疫反应中发挥重要作用。
