Sas D F, Lundy J K, Miller L J
Gastroenterology Research Unit, Mayo Clinic and Foundation, Rochester, Minnesota 55905.
In Vitro Cell Dev Biol. 1989 Aug;25(8):730-6. doi: 10.1007/BF02623726.
Due to limited growth potential of primary cultures and the absence of continuous lines of healthy enteric smooth muscle, we have studied the culture behavior of neoplastic gastrointestinal smooth muscle cells. Forty-six human enteric smooth muscle neoplasms (leiomyomas and leiomyosarcomas) were studied while fresh and/or after culture in vitro and growth in vivo in athymic nude mice, with assessments made of morphology, growth characteristics, and biochemical markers of differentiation. The state of differentiation of the tumors varied, with well-differentiated tumors tending to express binding sites for the gastrointestinal hormone cholecystokinin, whereas less well-differentiated tumors did not. Poorly differentiated tumors were the easiest to establish in culture in vitro and to grow in vivo in nude mice. When the cells placed directly into culture proliferated to confluent density, they underwent morphologic differentiation from a spread, fibroblastlike shape to a slender spindle morphology, with these cells possessing fewer biosynthetic organelles and arranging themselves in characteristic "hill and valley" arrays. However, the highly differentiated characteristics of expression of desmin or cholecystokinin-binding sites were not observed in cultured cells. In contrast, cells that had been passaged in nude mice before culture displayed a proliferative phenotype and failed to undergo morphologic differentiation on reaching confluent density. Four human enteric smooth muscle cell lines (documented by chromosomal analysis) originating in stomach, jejunum, ileum, and rectum were established using this strategy.
由于原代培养的生长潜力有限，且缺乏健康肠平滑肌的连续细胞系，我们研究了肿瘤性胃肠平滑肌细胞的培养行为。对46例人肠平滑肌肿瘤（平滑肌瘤和平滑肌肉瘤）进行了研究，观察其新鲜状态和/或体外培养后及在无胸腺裸鼠体内生长的情况，并对其形态、生长特性和分化的生化标志物进行了评估。肿瘤的分化状态各不相同，高分化肿瘤倾向于表达胃肠激素胆囊收缩素的结合位点，而低分化肿瘤则不表达。低分化肿瘤最容易在体外培养并在裸鼠体内生长。直接接种于培养基中的细胞增殖至汇合密度时，会发生形态分化，从铺展的、成纤维细胞样形态转变为细长的梭形形态，这些细胞的生物合成细胞器较少，并以特征性的“峰谷”排列方式排列。然而，在培养的细胞中未观察到结蛋白或胆囊收缩素结合位点表达的高分化特征。相反，在裸鼠体内传代后再进行培养的细胞表现出增殖表型，达到汇合密度时未发生形态分化。使用该策略建立了4株源自胃、空肠、回肠和直肠的人肠平滑肌细胞系（经染色体分析证实）。
