Graham M F, Diegelmann R F, Elson C O, Bitar K N, Ehrlich H P
Proc Soc Exp Biol Med. 1984 Sep;176(4):503-7. doi: 10.3181/00379727-176-4-rc1.
Intestinal smooth muscle cells were isolated from human bowel and maintained in culture through several passages. These cells were obtained by enzyme digestion of slices taken from the circular layer of the muscularis propria of human jejunum. When subcultured, they initially flattened out and then began proliferating after 3 days. After 3 weeks in culture, they began aggregating into ridges. Fluorohistochemical staining revealed numerous prominent actin stress fibers. When these cells were exposed to the C-terminal octapeptide of cholecystokinin they contracted in a dose-dependent fashion. The availability of human intestinal smooth muscle cells in culture will considerably enhance our ability to study the contractile, proliferative and connective tissue responses of the smooth muscle of the human gastrointestinal tract.
从人肠道分离出肠道平滑肌细胞，并通过多次传代在培养中维持。这些细胞是通过酶消化取自人空肠肌层固有环层的切片获得的。传代培养时，它们最初会变平，然后在3天后开始增殖。培养3周后，它们开始聚集成嵴。荧光组织化学染色显示有许多明显的肌动蛋白应力纤维。当这些细胞暴露于胆囊收缩素的C末端八肽时，它们会以剂量依赖性方式收缩。培养中的人肠道平滑肌细胞的可得性将大大增强我们研究人胃肠道平滑肌的收缩、增殖和结缔组织反应的能力。
