Lee Jun-Kyoung, Lee Soyoung, Baek Moon-Chang, Lee Byung-Heon, Lee Hyun-Shik, Kwon Taeg Kyu, Park Pil-Hoon, Shin Tae-Yong, Khang Dongwoo, Kim Sang-Hyun
CMRI, Departments of Pharmacology, Kyungpook National University, Daegu, Republic of Korea.
Natural Product Research Center, Research Institute of Bioscience and Biotechnology, Jeongeup, Republic of Korea.
J Appl Toxicol. 2017 May;37(5):554-562. doi: 10.1002/jat.3389. Epub 2016 Sep 29.
Perfluorooctanoic acid (PFOA) has wide applications, including as a raw material for converted paper and packaging products. With the widespread use of PFOA, concerns regarding its potential environmental and health impacts have increased. In spite of the known hepatotoxicity and genotoxicity of PFOA, correlation with PFOA and allergic inflammation is not well known. In this study, the effect of PFOA on the degranulation of mast cells and mast cell-mediated allergic inflammation in the presence of FcεRI cross-linking was evaluated. In immunoglobulin (Ig) E-stimulated mast cells, PFOA increased the release of histamine and β-hexosaminidase by the up-regulation of intracellular calcium levels. PFOA enhanced gene expression of several pro-inflammatory cytokines, including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, and IL-8 by the activation of nuclear factor (NF)-κB in IgE-stimulated mast cells. Also, PFOA exacerbated allergic symptoms via hypothermia, and an increase of serum histamine, TNF-α, IgE and IgG in the ovalbumin-induced systemic anaphylaxis. The present data indicate that PFOA aggravated FcɛRI-mediated mast cell degranulation and allergic symptoms. Copyright © 2016 John Wiley & Sons, Ltd.
全氟辛酸（PFOA）有广泛应用，包括作为转化纸和包装产品的原材料。随着PFOA的广泛使用，对其潜在环境和健康影响的担忧增加。尽管已知PFOA具有肝毒性和遗传毒性，但PFOA与过敏性炎症的相关性尚不明确。在本研究中，评估了PFOA在存在FcεRI交联的情况下对肥大细胞脱颗粒和肥大细胞介导的过敏性炎症的影响。在免疫球蛋白（Ig）E刺激的肥大细胞中，PFOA通过上调细胞内钙水平增加组胺和β-己糖胺酶的释放。PFOA通过激活IgE刺激的肥大细胞中的核因子（NF）-κB增强了几种促炎细胞因子的基因表达，包括肿瘤坏死因子（TNF）-α、白细胞介素（IL）-1β、IL-6和IL-8。此外，PFOA通过体温过低以及卵清蛋白诱导的全身过敏反应中血清组胺、TNF-α、IgE和IgG的增加加剧了过敏症状。目前的数据表明，PFOA加重了FcɛRI介导的肥大细胞脱颗粒和过敏症状。版权所有©2016约翰威立父子有限公司。
