Department of Immunology and Institute of Medical Sciences, Medical School, Jeonbuk (Chonbuk) National University, Jeonju, Jeonbuk 54907, Korea.
Department of Oriental Pharmacy, College of Pharmacy and Wonkwang-Oriental Medicines Research Institute, Wonkwang University, Iksan, Jeonbuk 54538, Korea.
Molecules. 2020 Sep 12;25(18):4178. doi: 10.3390/molecules25184178.
Mast cells are effector cells that initiate allergic inflammatory immune responses by inducing inflammatory mediators. (Linn.) Gaudich is a natural herb in the nettle family Urticaceae that possesses numerous pharmacological properties. Despite the various pharmacological benefits of , its effects on allergic inflammation have not yet been determined. Here, we investigated the effect of the ethanol extract of (BNE) on degranulation rat basophilic leukemia (RBL)-2H3 mast cells stimulated with anti-dinitrophenyl (anti-DNP) and bovine serum albumin (BSA) during immunoglobulin E (IgE)-mediated allergic immune response. The results showed inhibition of the release of β-hexosaminidase and histamine from the cells. BNE suppressed pro-inflammatory cytokines (Tumor necrosis factor (TNF)-α, Interleukin (IL)-1β, and IL-6) and reduced T helper (Th)2 cytokine IL-4 expression and/or secretion correlated with the downregulation of p38, extracellular signal-regulated kinases (ERK) mitogen-activated protein kinase (MAPK), and nuclear factor-κB (NF-κB) signaling pathways in treated RBL-2H3 mast cells. In passive cutaneous anaphylaxis, treatment with BNE during IgE-mediated local allergic reaction triggered a reduction in mouse ear pigmentation and thickness. Taken together, these results indicated that BNE suppressed mast cell-mediated inflammation, suggesting that BNE might be a candidate for the treatment of various allergic disorders.
肥大细胞是通过诱导炎症介质启动过敏炎症免疫反应的效应细胞。荨麻科荨麻属植物（Linn.）Gaudich 是一种天然草药，具有多种药理学特性。尽管 具有多种药理学益处，但它对过敏炎症的影响尚未确定。在这里，我们研究了乙醇提取物对 IgE 介导的过敏免疫反应中抗二硝基苯（anti-DNP）和牛血清白蛋白（BSA）刺激的大鼠嗜碱性白血病（RBL）-2H3 肥大细胞脱颗粒的影响。结果表明，它抑制了β-己糖胺酶和组胺从细胞中的释放。BNE 抑制了促炎细胞因子（肿瘤坏死因子 (TNF)-α、白细胞介素 (IL)-1β 和 IL-6）的表达，并减少了 Th2 细胞因子 IL-4 的表达和/或分泌，这与 p38、细胞外信号调节激酶 (ERK) 丝裂原活化蛋白激酶 (MAPK) 和核因子-κB (NF-κB) 信号通路的下调相关。在被动皮肤过敏反应中，在 IgE 介导的局部过敏反应期间用 BNE 治疗导致小鼠耳色素沉着和厚度减少。总之，这些结果表明 BNE 抑制了肥大细胞介导的炎症，表明 BNE 可能是治疗各种过敏疾病的候选药物。
