Department of Pharmacology, Faculty of Pharmacy and Pharmaceutical Sciences, College of Health Sciences, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana.
Pharmacology/Toxicology Department, Centre for Plant Medicine Research (CPMR), Mampong-Akuapem, Ghana.
BMC Complement Med Ther. 2022 Mar 19;22(1):80. doi: 10.1186/s12906-022-03565-6.
Holarrhena floribunda (G.Don) T.Durand & Schinz stem bark has anecdotal use in Ghanaian folk medicine for the management of inflammatory conditions. This study was conducted to investigate the in vivo anti-inflammatory activity of the bark extract using models of acute inflammation in male Sprague Dawley rats, C57BL/6 mice and ICR mice.
A 70% hydro-ethanol extract of the stem bark (HFE) was evaluated at doses of 5-500 mg/kg bw. Local anaphylaxis was modelled by the pinnal cutaneous anaphylactic test. Systemic anaphylaxis or sepsis were modeled by compound 48/80 or lipopolysaccharide, respectively. Clonidine-induced catalepsy was used to investigate the effect on histamine signaling. Anti-oedematogenic effect was assessed by induction with carrageenan. Effects on mediators of biphasic acute inflammation were studied using histamine and serotonin (early phase) or prostaglandin E2 (late phase).
HFE demonstrated anti-inflammatory and/or anti-oedematogenic activity comparable to standard doses of aspirin and diclofenac (inhibitors of cyclooxygenases-1 and -2), chlorpheniramine (histamine H1-receptor antagonist), dexamethasone (glucocorticoid receptor agonist), granisetron (serotonin receptor antagonist) and sodium cromoglycate (inhibitor of mast cell degranulation). All observed HFE bioactivities increased with dose.
The data provide evidence that the extract of H. floribunda stem bark has anti-anaphylactic and anti-oedematogenic effects; by interfering with signalling or metabolism of histamine, serotonin and prostaglandin E which mediate the progression of inflammation. The anti-inflammatory and antihistaminic activities of HFE may be relevant in the context of the management of COVID-19.
Holarrhena floribunda（G.Don）T.Durand & Schinz 茎皮在加纳民间医学中被用于治疗炎症性疾病，具有传闻证据。本研究旨在使用雄性 Sprague Dawley 大鼠、C57BL/6 小鼠和 ICR 小鼠的急性炎症模型，研究树皮提取物的体内抗炎活性。
用 70%水-乙醇提取物（HFE），剂量为 5-500mg/kg bw。用耳廓皮肤过敏性试验建立局部过敏反应模型。用化合物 48/80 或脂多糖分别建立全身性过敏反应或脓毒症模型。用氯苯那敏诱导的僵住实验研究对组胺信号的影响。用角叉菜胶诱导的水肿评估抗水肿作用。用组胺和 5-羟色胺（早期阶段）或前列腺素 E2（晚期阶段）研究对双相急性炎症介质的影响。
HFE 表现出与阿司匹林和双氯芬酸（环氧化酶-1 和 -2 的抑制剂）、氯苯那敏（组胺 H1 受体拮抗剂）、地塞米松（糖皮质激素受体激动剂）、格兰昔罗（5-羟色胺受体拮抗剂）和色甘酸钠（肥大细胞脱颗粒抑制剂）的标准剂量相当的抗炎和/或抗水肿活性。所有观察到的 HFE 生物活性均随剂量增加而增加。
这些数据提供了证据，表明 H. floribunda 茎皮提取物具有抗过敏和抗水肿作用；通过干扰组胺、5-羟色胺和前列腺素 E 的信号转导或代谢，这些物质介导炎症的进展。HFE 的抗炎和抗组胺活性可能与 COVID-19 的管理有关。
