Almeida-Dalmet Swati, Sikaroodi Masoumeh, Gillevet Patrick M, Litchfield Carol D, Baxter Bonnie K
Microbiome Analysis Center, Department of Environmental Science and Policy, George Mason University, 10900 University Blvd., Manassas, VA 20110, USA.
Great Salt Lake Institute, Westminster College, 1840 South 1300 East, Salt Lake City, UT 84105, USA.
Microorganisms. 2015 Jul 2;3(3):310-26. doi: 10.3390/microorganisms3030310.
We employed a temporal sampling approach to understand how the microbial diversity may shift in the north arm of Great Salt Lake, Utah, U.S. To determine how variations in seasonal environmental factors affect microbial communities, length heterogeneity PCR fingerprinting was performed using consensus primers for the domain Bacteria, and the haloarchaea. The archaeal fingerprints showed similarities during 2003 and 2004, but this diversity changed during the remaining two years of the study, 2005 and 2006. We also performed molecular phylogenetic analysis of the 16S rRNA genes of the whole microbial community to characterize the taxa in the samples. Our results indicated that in the domain, Bacteria, the Salinibacter group dominated the populations in all samplings. However, in the case of Archaea, as noted by LIBSHUFF for phylogenetic relatedness analysis, many of the temporal communities were distinct from each other, and changes in community composition did not track with environmental parameters. Around 20-23 different phylotypes, as revealed by rarefaction, predominated at different periods of the year. Some phylotypes, such as Haloquadradum, were present year-round although they changed in their abundance in different samplings, which may indicate that these species are affected by biotic factors, such as nutrients or viruses, that are independent of seasonal temperature dynamics.
我们采用了时间抽样方法来了解美国犹他州大盐湖北部臂中的微生物多样性是如何变化的。为了确定季节性环境因素的变化如何影响微生物群落，我们使用针对细菌域和嗜盐古菌的共有引物进行了长度异质性PCR指纹分析。古菌指纹在2003年和2004年显示出相似性，但在研究的其余两年（2005年和2006年）中这种多样性发生了变化。我们还对整个微生物群落的16S rRNA基因进行了分子系统发育分析，以表征样本中的分类群。我们的结果表明，在细菌域中，盐杆菌属在所有采样中占主导地位。然而，就古菌而言，正如LIBSHUFF用于系统发育相关性分析所指出的，许多时间上的群落彼此不同，群落组成的变化与环境参数并不相关。通过稀疏分析揭示，大约20 - 23种不同的系统型在一年中的不同时期占主导地位。一些系统型，如嗜盐四联球菌，全年都存在，尽管它们在不同采样中的丰度有所变化，这可能表明这些物种受到生物因素的影响，如营养物质或病毒，这些因素与季节性温度动态无关。
