Shevchuk T V, Zakharchenko N S, Tarlachkov S V, Furs O V, Dyachenko O V, Buryanov Y I
Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Moscow Region, 142290, Russia.
Biochemistry (Mosc). 2016 Sep;81(9):968-71. doi: 10.1134/S0006297916090054.
Transgenic kalanchoe plants (Kalanchoe pinnata L.) expressing the antimicrobial peptide cecropin P1 gene (cecP1) under the control of the 35S cauliflower mosaic virus 35S RNA promoter and the selective neomycin phosphotransferase II (nptII) gene under the control of the nopaline synthase gene promoter were studied. The 35S promoter methylation and the cecropin P1 biosynthesis levels were compared in plants growing on media with and without kanamycin. The low level of active 35S promoter methylation further decreases upon cultivation on kanamycin-containing medium, while cecropin P1 synthesis increases.
对在花椰菜花叶病毒35S RNA启动子控制下表达抗菌肽天蚕素P1基因（cecP1）以及在胭脂碱合成酶基因启动子控制下表达选择性新霉素磷酸转移酶II（nptII）基因的转基因落地生根植物（Kalanchoe pinnata L.）进行了研究。比较了在含有和不含卡那霉素的培养基上生长的植物中35S启动子甲基化水平和天蚕素P1生物合成水平。在含卡那霉素的培养基上培养时，活性35S启动子的低甲基化水平进一步降低，而天蚕素P1的合成增加。
