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转基因协同抗菌肽 Cecropin P1 的杀菌、免疫调节和伤口愈合特性的体内研究。

Bactericide, Immunomodulating, and Wound Healing Properties of Transgenic Synergize with Antimicrobial Peptide Cecropin P1 In Vivo.

机构信息

Russian Academy of Sciences, Branch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Pushchino, Moscow Region, Russia.

Kursk State University, Kursk, Russia; Kursk State Medical University, Kursk, Russia.

出版信息

J Immunol Res. 2017;2017:4645701. doi: 10.1155/2017/4645701. Epub 2017 Feb 23.

DOI:10.1155/2017/4645701
PMID:28326334
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5343256/
Abstract

Procedure of manufacturing water extracts containing cecropin P1 (CecP1) from the formerly described transgenic plants is established. It included incubation of leaves at +4°C for 7 days, mechanical homogenization of leaves using water as extraction solvent, and heating at +70°C for inactivating plant enzymes. Yield of CecP1 (after heating and sterilizing filtration) was 0.3% of total protein in the extract. The water extract of + CecP1 exhibits favorable effect on healing of wounds infected with (equal to Cefazolin) and with a combination of with (better than Cefazolin). Wild-type extract exhibited evident microbicide activity against with but it was substantially strengthened in + CecP1 extract. extracts (both wild-type and transgenic) did not exhibit general toxicity and accelerated wound recovery. Due to immunomodulating activity, wild-type extract accelerated granulation of the wound bed and marginal epithelialization even better than + CecP1 extract. Immunomodulating and microbicide activity of synergizes with microbicide activity of CecP1 accelerating elimination of bacteria.

摘要

建立了从前述转基因植物中提取含有 Cecropin P1 (CecP1) 的水提取物的方法。该方法包括将叶片在+4°C下孵育 7 天，用水作为提取溶剂机械匀浆，以及在+70°C下加热以灭活植物酶。CecP1（加热和消毒过滤后）的产量为提取物中总蛋白的 0.3%。含有 CecP1 的水提取物对感染（相当于头孢唑林）和感染（优于头孢唑林）的伤口愈合具有良好的效果。野生型提取物对 具有明显的杀菌活性，但在含有 CecP1 的提取物中，其杀菌活性显著增强。野生型和转基因 提取物均没有表现出一般毒性，且加速了伤口的恢复。由于具有免疫调节活性，野生型提取物甚至比含有 CecP1 的提取物更能加速创面肉芽组织的形成和边缘上皮化。与 CecP1 的杀菌活性协同作用，增强了 的免疫调节和杀菌活性，加速了细菌的消除。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/327135fede9e/JIR2017-4645701.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/b0572a815cd2/JIR2017-4645701.001.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/cb70953536fb/JIR2017-4645701.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/3e3fecf04580/JIR2017-4645701.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/2ccc6cf7b85a/JIR2017-4645701.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/efd72a32d304/JIR2017-4645701.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/1a5fcfd20178/JIR2017-4645701.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/327135fede9e/JIR2017-4645701.008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/b0572a815cd2/JIR2017-4645701.001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/18c58fc618ca/JIR2017-4645701.002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/cb70953536fb/JIR2017-4645701.003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/3e3fecf04580/JIR2017-4645701.004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/2ccc6cf7b85a/JIR2017-4645701.005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/efd72a32d304/JIR2017-4645701.006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/1a5fcfd20178/JIR2017-4645701.007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2e26/5343256/327135fede9e/JIR2017-4645701.008.jpg

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