一种在大肠杆菌中检测抑制子的简单技术。
A simple technique for suppressor detection in Escherichia coli.
作者信息
Vinué Laura, Hooper David C
出版信息
FEMS Microbiol Lett. 2016 Oct 1;363(19). doi: 10.1093/femsle/fnw228.
Abstract
To study the viability of a gyrA S83 stop mutation found in an Escherichia coli J53 ciprofloxacin-resistant strain (J53 CipR27), a pBR322 derivative was constructed with a TAG mutation in the bla gene knocking out ampicillin resistance. Ampicillin resistance was restored, suggesting that the strain contains tRNA suppressor activity able to suppress the UAG codon gyrA and allow viability. The method was applied to 22 unique clinical E. coli isolates, and all were found to have low-level suppressor activity.
摘要
为研究在大肠杆菌J53环丙沙星耐药菌株（J53 CipR27）中发现的gyrA S83终止突变的生存能力，构建了一种pBR322衍生物，其bla基因存在TAG突变，从而敲除氨苄青霉素抗性。氨苄青霉素抗性得以恢复，这表明该菌株含有能够抑制UAG密码子gyrA并允许其生存的tRNA抑制活性。该方法应用于22株独特的临床大肠杆菌分离株，发现它们均具有低水平的抑制活性。
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