高效液相色谱法测定人血清中牛磺结合型和甘氨结合型胆汁酸
High-performance liquid chromatographic determination of tauro- and glyco-conjugated bile acids in human serum.
作者信息
Labbé D, Gerhardt M F, Myara A, Vercambre C, Trivin F
机构信息
Hôpital Saint-Joseph, Laboratoire de Biochimie, Paris, France.
出版信息
J Chromatogr. 1989 May 30;490(2):275-84. doi: 10.1016/s0378-4347(00)82785-1.
A method for the identification and individual determination of the ten tauro- and glyco-conjugated bile acids is described. It consists in a specific three-step extraction from small serum samples (500 microliters), high-performance liquid chromatographic separation and direct spectrophotometric detection at 119 nm. Extraction can be checked by the use of an internal standard. The reproducibility, recovery and separation of fractions were satisfactory.
描述了一种鉴定和单独测定十种牛磺和糖缀合胆汁酸的方法。它包括从小血清样本（500微升）中进行特定的三步提取、高效液相色谱分离以及在119纳米处直接进行分光光度检测。提取可通过使用内标进行检查。各组分的重现性、回收率和分离效果令人满意。
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