Edginton Ryan S, Mattana Sara, Caponi Silvia, Fioretto Daniele, Green Ellen, Winlove C Peter, Palombo Francesca
School of Physics and Astronomy, University of Exeter.
Department of Physics and Geology, University of Perugia.
J Vis Exp. 2016 Sep 15(115):54648. doi: 10.3791/54648.
Brillouin spectroscopy is an emerging technique in the biomedical field. It probes the mechanical properties of a sample through the interaction of visible light with thermally induced acoustic waves or phonons propagating at a speed of a few km/sec. Information on the elasticity and structure of the material is obtained in a nondestructive contactless manner, hence opening the way to in vivo applications and potential diagnosis of pathology. This work describes the application of Brillouin spectroscopy to the study of biomechanics in elastin and trypsin-digested type I collagen fibers of the extracellular matrix. Fibrous proteins of the extracellular matrix are the building blocks of biological tissues and investigating their mechanical and physical behavior is key to establishing structure-function relationships in normal tissues and the changes which occur in disease. The procedures of sample preparation followed by measurement of Brillouin spectra using a reflective substrate are presented together with details of the optical system and methods of spectral data analysis.
布里渊光谱学是生物医学领域中一种新兴的技术。它通过可见光与以每秒几千米速度传播的热致声波或声子的相互作用来探测样品的力学性能。以非破坏性、非接触的方式获取有关材料弹性和结构的信息，从而为体内应用和病理学的潜在诊断开辟了道路。这项工作描述了布里渊光谱学在研究细胞外基质中弹性蛋白和胰蛋白酶消化的I型胶原纤维生物力学方面的应用。细胞外基质的纤维蛋白是生物组织的基本组成部分，研究它们的力学和物理行为是建立正常组织中结构 - 功能关系以及疾病中发生变化的关键。本文介绍了样品制备程序，随后使用反射基板测量布里渊光谱，以及光学系统的详细信息和光谱数据分析方法。
