Ricietto Ana Paula Scaramal, Gomis-Cebolla Joaquín, Vilas-Bôas Gislayne Trindade, Ferré Juan
Departamento de Biologia Geral, Universidade Estadual de Londrina, Londrina, Paraná, Brazil; ERI de Biotecnología y Biomedicina (BIOTECMED), Department of Genetics, Universitat de València, 46100 Burjassot, Spain.
ERI de Biotecnología y Biomedicina (BIOTECMED), Department of Genetics, Universitat de València, 46100 Burjassot, Spain.
J Invertebr Pathol. 2016 Nov;141:1-5. doi: 10.1016/j.jip.2016.09.006. Epub 2016 Sep 26.
The Oriental fruit moth, Grapholita molesta (Lepidoptera: Tortricidae), is a major pest of fruit trees worldwide, such as peach and apple. Bacillus thuringiensis has been shown to be an efficient alternative to synthetic insecticides in the control of many agricultural pests. The objective of this study was to evaluate the effectiveness of B. thuringiensis individual toxins and their mixtures for the control of G. molesta. Bioassays were performed with Cry1Aa, Cry1Ac, Cry1Ca, Vip3Aa, Vip3Af and Vip3Ca, as well as with the commercial products DiPel® and XenTari®. The most active proteins were Vip3Aa and Cry1Aa, with LC values of 1.8 and 7.5ng/cm, respectively. Vip3Ca was nontoxic to this insect species. Among the commercial products, DiPel® was slightly, but significantly, more toxic than XenTari®, with LC values of 13 and 33ng commercial product/cm, respectively. Since Vip3A and Cry1 proteins are expressed together in some insect-resistant crops, we evaluated possible synergistic or antagonistic interactions among them. The results showed moderate to high antagonism in the combinations of Vip3Aa with Cry1Aa and Cry1Ca.
桃小食心虫，即桃蛀果蛾（Grapholita molesta，鳞翅目：卷蛾科），是全球范围内果树的主要害虫，如桃树和苹果树。苏云金芽孢杆菌已被证明是控制许多农业害虫时替代合成杀虫剂的有效选择。本研究的目的是评估苏云金芽孢杆菌单个毒素及其混合物对桃小食心虫的防治效果。使用Cry1Aa、Cry1Ac、Cry1Ca、Vip3Aa、Vip3Af和Vip3Ca以及商业产品敌宝（DiPel®）和赛灭灵（XenTari®）进行生物测定。活性最高的蛋白是Vip3Aa和Cry1Aa，其LC值分别为1.8和7.5纳克/平方厘米。Vip3Ca对该昆虫种类无毒。在商业产品中，敌宝（DiPel®）的毒性略高于赛灭灵（XenTari®），但差异显著，其LC值分别为13和33纳克商业产品/平方厘米。由于Vip3A和Cry1蛋白在一些抗虫作物中共同表达，我们评估了它们之间可能存在 的协同或拮抗相互作用。结果表明，Vip3Aa与Cry1Aa和Cry1Ca的组合存在中度到高度的拮抗作用。
