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TOXiTAXi：一个关于苏云金芽孢杆菌蛋白组合物对不同分类群物种毒性的网络资源。

TOXiTAXi: a web resource for toxicity of Bacillus thuringiensis protein compositions towards species of various taxonomic groups.

机构信息

Department of Microbiology, Faculty of Biology, Adam Mickiewicz University in Poznan, Uniwersytetu Poznanskiego 6, 61-614, Poznan, Poland.

Department of Computational Biology, Faculty of Biology, Adam Mickiewicz University in Poznan, Uniwersytetu Poznanskiego 6, 61-614, Poznan, Poland.

出版信息

Sci Rep. 2020 Nov 13;10(1):19767. doi: 10.1038/s41598-020-75932-7.

DOI:10.1038/s41598-020-75932-7
PMID:33188218
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7666212/
Abstract

Bioinsecticides consisting of different sets of Bacillus thuringiensis (Bt) Cry, Cyt and Vip toxins are broadly used in pest control. Possible interactions (synergistic, additive or antagonistic) between these proteins can not only influence the overall efficacy of certain Bt-based bioinsecticide, but also raise questions regarding environmental safety. Here, we assemble, summarize and analyze the outcomes of experiments published over 30 years, investigating combinatorial effects among Bt Cry, Cyt and Vip toxins. We collected the results on 118 various two-to-five-component combinations that have been bioassayed against 38 invertebrate species. Synergism, additive effect and antagonism was indicated in 54%, 32% and 14% of experiments, respectively. Synergism was noted most frequently for Cry/Cyt combinations, followed by Cyt/Vip and Cry/Cry. In Cry/Vip combinations, antagonism is more frequent and higher in magnitude compared to other categories. Despite a significant number of tested Bt toxin combinations, most of them have been bioassayed only against one pest species. To aid the research on Bt pesticidal protein activity, we present TOXiTAXi ( http://www.combio.pl/toxitaxi/ ), a universal database and a dedicated web tool to conveniently gather and analyze the existing and future bioassay results on biocidal activity of toxins against various taxonomic groups.

摘要

由不同的苏云金芽孢杆菌 (Bt) Cry、Cyt 和 Vip 毒素组成的生物杀虫剂被广泛用于害虫防治。这些蛋白质之间可能存在相互作用（协同、相加或拮抗），这不仅会影响某些基于 Bt 的生物杀虫剂的整体效果，还会引发关于环境安全性的问题。在这里，我们收集、总结和分析了 30 多年来发表的实验结果，研究了 Bt Cry、Cyt 和 Vip 毒素之间的组合效应。我们收集了针对 38 种无脊椎动物物种进行生物测定的 118 种不同的两到五组分组合的结果。协同作用、相加效应和拮抗作用分别在 54%、32%和 14%的实验中得到了证实。Cry/Cyt 组合最常出现协同作用，其次是 Cyt/Vip 和 Cry/Cry。在 Cry/Vip 组合中，与其他类别相比，拮抗作用更频繁且强度更高。尽管已经测试了大量的 Bt 毒素组合，但其中大多数仅针对一种害虫物种进行了生物测定。为了帮助研究 Bt 杀虫蛋白的活性，我们提供了 TOXiTAXi（http://www.combio.pl/toxitaxi/），这是一个通用数据库和专用网络工具，用于方便地收集和分析针对各种分类群的毒素生物杀灭活性的现有和未来生物测定结果。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/1e3a81656693/41598_2020_75932_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/9a71cc65d140/41598_2020_75932_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/71a628135575/41598_2020_75932_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/c99bf3e08df1/41598_2020_75932_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/72af0bdd9aa0/41598_2020_75932_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/1e3a81656693/41598_2020_75932_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/9a71cc65d140/41598_2020_75932_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/71a628135575/41598_2020_75932_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/c99bf3e08df1/41598_2020_75932_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/72af0bdd9aa0/41598_2020_75932_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/6429/7666212/1e3a81656693/41598_2020_75932_Fig5_HTML.jpg

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