Departamento de Genética/ERI BioTecMed, Universitat de València, Burjassot, 46100 València, Spain.
Department of Plant Protection, College of Agriculture and Natural Resources, University of Tehran, Karaj 31578-77871, Alborz, Iran.
Toxins (Basel). 2020 Oct 26;12(11):673. doi: 10.3390/toxins12110673.
Discovery and identification of novel insecticidal proteins in (Bt) strains are of crucial importance for efficient biological control of pests and better management of insect resistance. In this study, the Bt strain KhF, toxic for and larvae, underwent genomics and proteomics analyses to achieve a better understanding of the bases of its pathogenicity. The whole-genome sequencing results revealed that the KhF strain contained nine coding sequences with homologies to Bt insecticidal genes. The lepidopteran toxic mixture of spores and crystals of this Bt strain was subjected to liquid chromatography and tandem mass spectrometry (LC-MS/MS) to assess the protein composition. The results of the proteomic analyses, combined with the toxin gene sequences, revealed that two of the main components of the crystals were two new candidate pesticidal proteins, named KhFA and KhFB. These proteins showed a similarity lower than 36% to the other known Bt toxins. The phylogenetic analysis showed that the KhFA and KhFB grouped with the newly denominated Xpp and Mpp (former ETX/Mtx) pesticidal protein groups, respectively. Altogether, this study has led to the discovery of two novel candidate pesticidal toxins in the lepidopteran toxic KhF strain.
发现和鉴定新型杀虫蛋白在（Bt）菌株中对于害虫的有效生物防治和更好地管理昆虫抗性至关重要。在这项研究中，对杀虫的KhF 菌株进行了基因组学和蛋白质组学分析，以更好地了解其致病基础。全基因组测序结果表明，KhF 菌株含有九个与 Bt 杀虫基因同源的编码序列。该 Bt 菌株的鳞翅目毒混合孢子和晶体进行了液相色谱和串联质谱（LC-MS/MS）分析，以评估蛋白质组成。蛋白质组学分析的结果与毒素基因序列相结合，表明晶体的两个主要成分是两种新的候选杀虫剂蛋白，分别命名为 KhFA 和 KhFB。这些蛋白与其他已知的 Bt 毒素的相似性低于 36%。系统发育分析表明，KhFA 和 KhFB 分别与新命名的 Xpp 和 Mpp（前 ETX/Mtx）杀虫蛋白组聚在一起。总的来说，这项研究导致在鳞翅目毒 KhF 菌株中发现了两种新型候选杀虫剂毒素。
