Zhu S F, Li J G
Department of Intensive Care Unit, Zhongnan Hospital of Wuhan University, Wuhan 430071, China.
Zhonghua Yi Xue Za Zhi. 2016 Sep 20;96(35):2791-2795. doi: 10.3760/cma.j.issn.0376-2491.2016.35.006.
To investigate the different genotype and allele frequency distribution of ADAM33 gene T1, T2, V4, S2 sites Mongolian population, and discuss the relationship between ADAM33 gene polymorphism and bronchial asthma. From January 2014 to December 2015, a total of 180 cases of Mongolian patients with asthma were detected, compared with 186 cases of healthy Mongolian as controled and screening significant genes.Selected restriction fragment length polymorphism (PCR-RFLP) method to detected ADAM33 gene polymorphism.According to condition , the asthma group was divided into mild(=83), medium group(=47)and severe group(=50). The distribution difference of different genotype and every genotype of V4 FEV1, eosinophils, IgE comparison were compared , and analysis their correlation. In ADAM33 , the distribution of T1 sites (AA and AG genotypes) had statistical significance compared asthma group with control group(χ=8.810, 8.294, <0.05, =1.983, 0.500). The value of G allele was 0.580.The distribution of S2 site(CC genotype) had statistical significance(χ=4.277, <0.05), the value of G allele was 1.423.the distribution of V4 sites (GC and GG genotypes) had statistical significance between the two groups (χ=7.880, 10.313, <0.05), value was 0.459, 2.130, G allele value was 1.496.The distribution frequency difference of each genotype in V4 sites in mild, medium and severe group was statistically significant (χ=16.049, <0.05), and compared various genotypes of FEV1, IgE, the difference was statistically significant (<0.05), for each genotype of T2 site in asthma group and the control group there was no statistically significant in the distribution (χ=1.218, 0.248, 1.287, >0.05). T1, V4, S2 locus polymorphism of ADAM33 gene may play a role in the Mongolian asthma population, and T2 locus polymorphism may has no relationship with Mongolian asthma patients.And the genotype polymorphism of V4 sites may be associated with asthma severity.
为探讨ADAM33基因T1、T2、V4、S2位点在蒙古族人群中的不同基因型及等位基因频率分布情况，并探讨ADAM33基因多态性与支气管哮喘的关系。2014年1月至2015年12月，共检测180例蒙古族哮喘患者，以186例健康蒙古族作为对照，筛选相关基因。采用限制性片段长度多态性聚合酶链反应（PCR-RFLP）方法检测ADAM33基因多态性。根据病情，哮喘组分为轻度（=83）、中度（=47）和重度（=50）组。比较不同基因型及V4位点各基因型的FEV1、嗜酸性粒细胞、IgE分布差异，并分析其相关性。在ADAM33基因中，哮喘组与对照组比较，T1位点（AA和AG基因型）分布有统计学意义（χ=8.810，8.294，<0.05，=1.983，0.500）。G等位基因频率值为0.580。S2位点（CC基因型）分布有统计学意义（χ=4.277，<0.05），G等位基因频率值为1.423。两组间V4位点（GC和GG基因型）分布有统计学意义（χ=7.880，10.313，<0.05），频率值为0.459，2.130，G等位基因频率值为1.496。轻度、中度和重度组V4位点各基因型分布频率差异有统计学意义（χ=16.049，<0.05），比较各基因型的FEV1、IgE，差异有统计学意义（<0.05），哮喘组与对照组T2位点各基因型分布无统计学意义（χ=1.218，0.248，1.287，>0.05）。ADAM33基因的T1、V4、S2位点多态性可能在蒙古族哮喘人群中起作用，而T2位点多态性可能与蒙古族哮喘患者无关。V4位点的基因型多态性可能与哮喘严重程度相关。
