Zhu Shufen, Li Pengfei, Suo Hong, Dong Jingsheng, Cui Liying
Affiliated Hospital of Inner Mongolia Medical University, Hohhot 010011, China.
Inner Mongolia Autonomous Region People's Hospital, Hohhot 010011, China.
Saudi J Biol Sci. 2018 Dec;25(8):1795-1799. doi: 10.1016/j.sjbs.2018.08.018. Epub 2018 Aug 18.
Polymorphisms in the gene encoding for A disintegrin and metalloprotease 33 (ADAM33) are closely associated with the risk of bronchial asthma attacks in different populations. We collected blood samples from 248 asthma patients - 130 of the Han ethnic group and 118 of the Mongolian ethnic group - living in the Inner Mongolia region of China, and analyzed the single nuclear polymorphisms (SNPs) of the T1, T2 and V4 loci of the ADAM33 gene using PCR-RFLP (restriction fragment length polymorphism). In addition, we also tested 256 healthy controls (134 and 122 from the Han and Mongolian ethnic groups respectively) for the same SNPs. Three genotypes of the T1, T2 and V4 loci were predominantly detected: while polymorphisms in the T1 locus were significantly associated with asthma risk in both Mongolian and Han ethnicities ( < 0.05, < 0.05), that in the V4 locus were relevant only in the Mongolian patients ( < 0.05, > 0.05). In contrast, polymorphisms in the T2 locus showed no significant association with asthma risk in either ethnic group ( > 0.05, > 0.05).
解整合素金属蛋白酶33（ADAM33）编码基因的多态性与不同人群支气管哮喘发作风险密切相关。我们收集了居住在中国内蒙古地区的248例哮喘患者的血样，其中汉族130例，蒙古族118例，并采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术分析了ADAM33基因T1、T2和V4位点的单核甘酸多态性（SNP）。此外，我们还对256名健康对照者（汉族134例，蒙古族122例）进行了相同SNP的检测。主要检测到T1、T2和V4位点的三种基因型：T1位点的多态性在蒙古族和汉族中均与哮喘风险显著相关（P<0.05，P<0.05），而V4位点的多态性仅在蒙古族患者中具有相关性（P<0.05，P>0.05）。相比之下，T2位点的多态性在两个民族中均与哮喘风险无显著关联（P>0.05，P>0.05）。
