Triadó-Margarit X, Veillette M, Duchaine C, Talbot M, Amato F, Minguillón M C, Martins V, de Miguel E, Casamayor E O, Moreno T
Centre for Advanced Studies of Blanes (CEAB), Spanish Research Council (CSIC), Blanes, Spain.
Centre de Recherche de l'Institut Universitaire de Cardiologie et de Pneumologie de Québec, Québec, QC, Canada.
Indoor Air. 2017 May;27(3):564-575. doi: 10.1111/ina.12343. Epub 2016 Oct 26.
Subway systems worldwide transport more than 100 million people daily; therefore, air quality on station platforms and inside trains is an important urban air pollution issue. We examined the microbiological composition and abundance in space and time of bioaerosols collected in the Barcelona subway system during a cold period. Quantitative PCR was used to quantify total bacteria, Aspergillus fumigatus, influenza A and B, and rhinoviruses. Multitag 454 pyrosequencing of the 16S rRNA gene was used to assess bacterial community composition and biodiversity. The results showed low bioaerosol concentrations regarding the targeted microorganisms, although the bacterial bioburden was rather high (10 bacteria/m ). Airborne bacterial communities presented a high degree of overlap among the different subway environments sampled (inside trains, platforms, and lobbies) and were dominated by a few widespread taxa, with Methylobacterium being the most abundant genus. Human-related microbiota in sequence dataset and ascribed to potentially pathogenic bacteria were found in low proportion (maximum values below 2% of sequence readings) and evenly detected. Hence, no important biological exposure marker was detected in any of the sampled environments. Overall, we found that commuters are not the main source of bioaerosols in the Barcelona subway system.
全球地铁系统每日运送乘客超过1亿人次；因此，地铁站台和列车车厢内的空气质量是一个重要的城市空气污染问题。我们研究了寒冷时期巴塞罗那地铁系统中采集的生物气溶胶在空间和时间上的微生物组成及丰度。采用定量PCR对总细菌、烟曲霉、甲型和乙型流感病毒以及鼻病毒进行定量。利用16S rRNA基因的多标签454焦磷酸测序评估细菌群落组成和生物多样性。结果显示，尽管细菌生物负荷相当高（10⁶ 个细菌/立方米），但目标微生物的生物气溶胶浓度较低。在不同的地铁环境（列车车厢内、站台和大厅）中采集的空气传播细菌群落呈现出高度重叠，且由少数广泛分布的分类群主导，其中甲基杆菌属是最丰富的属。在序列数据集中与人类相关的微生物群以及归因于潜在致病细菌的微生物群比例较低（最大值低于序列读数的2%）且分布均匀。因此，在任何采样环境中均未检测到重要的生物暴露标志物。总体而言，我们发现通勤者并非巴塞罗那地铁系统中生物气溶胶的主要来源。
