Erbasan Funda, Alikanoğlu Arsenal Sezgin, Yazısız Veli, Karasu Uğur, Balkarlı Ayşe, Sezer Cem, Terzioğlu Mustafa Ender
Department of Rheumatology, Antalya Training and Research Hospital, Antalya, Turkey.
Department of Pathology, Antalya Training and Research Hospital, Antalya, Turkey.
Pathol Res Pract. 2016 Nov;212(11):1010-1014. doi: 10.1016/j.prp.2016.08.009. Epub 2016 Sep 2.
The role of leptin in primary Sjögren's syndrome (SS) pathogenesis is unknown. The aim of this study was to investigate the expression of leptin and leptin receptor (LEPR) in minor salivary glands in patients with SS.
The expression of leptin and LEPR in minor salivary gland specimens obtained from patients with primary SS (n=50) and control subjects (n=50) were examined using immunohistochemical staining.
Acinar cells, epithelial cells and adipocytes in salivary glands can express leptin and LEPR. It was observed that there was intense staining in the focal lymphocytic infiltration areas in SS patients. The intensity of leptin and LEPR staining under microscopy (400×) were graded semiquantitatively as negative, mild, moderate or strongly positive, and scored as 1, 2 or 3, respectively. The expression levels of leptin and LEPR in patients with primary SS were not higher than in controls. There was no significant difference in degrees of leptin and LEPR staining, staining intensity, and immunoreactive scores between groups. The expression of leptin and LEPR were not correlated with autoantibodies such as RF, ANA, anti-Ro, and/or anti-La positivity.
These findings indicate that leptin and its receptors do not play an important role in primary SS pathophysiology.
瘦素在原发性干燥综合征（SS）发病机制中的作用尚不清楚。本研究旨在调查SS患者小唾液腺中瘦素和瘦素受体（LEPR）的表达情况。
采用免疫组织化学染色法检测从原发性SS患者（n = 50）和对照受试者（n = 50）获取的小唾液腺标本中瘦素和LEPR的表达。
唾液腺中的腺泡细胞、上皮细胞和脂肪细胞均可表达瘦素和LEPR。观察到SS患者的局灶性淋巴细胞浸润区域有强烈染色。在显微镜下（400×），瘦素和LEPR染色强度半定量分为阴性、轻度、中度或强阳性，分别记为1、2或3分。原发性SS患者中瘦素和LEPR的表达水平不高于对照组。两组之间瘦素和LEPR染色程度、染色强度及免疫反应评分无显著差异。瘦素和LEPR的表达与RF、ANA、抗Ro和/或抗La等自身抗体阳性无关。
这些发现表明瘦素及其受体在原发性SS病理生理过程中不发挥重要作用。
