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厌氧侧流反应器中微生物群落结构因厌氧固体停留时间和污泥交换率变化而发生变化。

Shift in microbial community structure of anaerobic side-stream reactor in response to changes to anaerobic solid retention time and sludge interchange ratio.

机构信息

Department of Civil, Environmental and Mechanical Engineering, University of Trento, Trento, Italy.

Department of Civil, Environmental and Mechanical Engineering, University of Trento, Trento, Italy.

出版信息

Bioresour Technol. 2016 Dec;221:588-597. doi: 10.1016/j.biortech.2016.09.077. Epub 2016 Sep 22.

DOI:10.1016/j.biortech.2016.09.077
PMID:27689352
Abstract

A laboratory scale nutrient removal activated sludge system coupled with an anaerobic side-stream reactor was operated for 300 days treating real urban wastewater. A significant decrease in sludge production was obtained increasing the anaerobic solid retention time (SRT) and decreasing the sludge interchange ratio (IR). In this study, the microbial community structure was analyzed and compared with the sludge reduction performance. Quantitative polymerase chain reaction analyses encoding 16 ribosomal RNA and functional genes revealed a wide diversity of phylogenetic groups in each experimental period, resulting from long solids retention time and recirculation of sludge under aerobic, anoxic and anaerobic conditions. However, decreasing SRT from 10 to 2.5d and increasing IR from 27 to 100%, an increasing selection of both fermenting bacteria able to release extracellular polymeric substances and hydrolyze organic matter and slow growing bacteria involved in nutrient removal were detected and linked to the sludge reduction mechanisms.

摘要

采用实验室规模的营养物去除活性污泥系统与厌氧侧流反应器相耦合,处理实际城市污水 300 天。通过增加厌氧固体停留时间 (SRT) 和降低污泥交换率 (IR),可显著减少污泥产量。在本研究中,分析了微生物群落结构,并将其与污泥减少性能进行了比较。定量聚合酶链反应分析编码 16 核糖体 RNA 和功能基因,揭示了每个实验期间存在广泛的系统发育群多样性,这是由于长固体停留时间和在好氧、缺氧和厌氧条件下污泥的再循环。然而,当 SRT 从 10 天降至 2.5 天,IR 从 27 增加至 100%时,检测到既能释放细胞外聚合物物质和水解有机物,又能进行发酵的细菌以及参与营养物去除的生长缓慢的细菌的选择压力增加,这与污泥减少机制有关。

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