Traceability of Satsuma Mandarin (Citrus unshiu Marc.) Honey through Nectar/Honey-Sac/Honey Pathways of the Headspace, Volatiles, and Semi-Volatiles: Chemical Markers.

Headspace solid-phase microextraction (HS-SPME) and ultrasonic solvent extraction (USE), followed by GC-MS/FID, were applied for monitoring the nectar (NE)/honey-sac (HoS)/honey (HO) pathways of the headspace, volatiles, and semi-volatiles. The major NE (4 varieties of ) headspace compounds were linalool, α-terpineol, 1-indole, methyl anthranilate, and phenylacetonitrile. Corresponding extracts contained, among others, 1-indole, methyl anthranilate, 1,3-dihydro-2-indol-2-one and caffeine. The major HoS headspace compounds were linalool, α-terpineol, 1,8-cineole, 1-indole, methyl anthranilate, and -jasmone. Characteristic compounds from HoS extract were caffeine, 1-indole, 1,3-dihydro-2-indol-2-one, methyl anthranilate, and phenylacetonitrile. However, HO headspace composition was significantly different in comparison to NE and HoS with respect to phenylacetaldehyde and linalool derivatives abundance that appeared as the consequence of the hive conditions and the bee enzyme activity. honey traceability is determined by chemical markers: phenylacetaldehyde, phenylacetonitrile, linalool and its derivatives, as well as 1-indole, 1,3-dihydro-2-indol-2-one, and caffeine.