Carvalho Fabiana M C, Lima Vanessa C O, Costa Izael S, Medeiros Amanda F, Serquiz Alexandre C, Lima Maíra C J S, Serquiz Raphael P, Maciel Bruna L L, Uchôa Adriana F, Santos Elizeu A, Morais Ana H A
Postgraduate Nutrition Program, Center for Health Sciences, Federal University of Rio Grande do Norte, Natal RN 59078-970, Brazil.
Postgraduate Biochemistry Program, Biosciences Center, Federal University of Rio Grande do Norte, Natal RN 59078-970, Brazil.
Nutrients. 2016 Sep 27;8(10):544. doi: 10.3390/nu8100544.
Trypsin inhibitors are studied in a variety of models for their anti-obesity and anti-inflammatory bioactive properties. Our group has previously demonstrated the satietogenic effect of tamarind seed trypsin inhibitors (TTI) in eutrophic mouse models and anti-inflammatory effects of other trypsin inhibitors. In this study, we evaluated TTI effect upon satiety, biochemical and inflammatory parameters in an experimental model of metabolic syndrome (MetS). Three groups of = 5 male rats with obesity-based MetS received for 10 days one of the following: (1) Cafeteria diet; (2) Cafeteria diet + TTI (25 mg/kg); and (3) Standard diet. TTI reduced food intake in animals with MetS. Nevertheless, weight gain was not different between studied groups. Dyslipidemia parameters were not different with the use of TTI, only the group receiving standard diet showed lower very low density lipoprotein (VLDL) and triglycerides (TG) (Kruskal-Wallis, < 0.05). Interleukin-6 (IL-6) production did not differ between groups. Interestingly, tumor necrosis factor-alpha (TNF-α) was lower in animals receiving TTI. Our results corroborate the satietogenic effect of TTI in a MetS model. Furthermore, we showed that TTI added to a cafeteria diet may decrease inflammation regardless of weight loss. This puts TTI as a candidate for studies to test its effectiveness as an adjuvant in MetS treatment.
人们在各种模型中研究了胰蛋白酶抑制剂的抗肥胖和抗炎生物活性。我们小组之前已在营养正常的小鼠模型中证明了罗望子种子胰蛋白酶抑制剂（TTI）的促饱腹感作用以及其他胰蛋白酶抑制剂的抗炎作用。在本研究中，我们在代谢综合征（MetS）实验模型中评估了TTI对饱腹感、生化和炎症参数的影响。三组基于肥胖的患有MetS的雄性大鼠，每组n = 5，连续10天接受以下处理之一：（1）自助餐饮食；（2）自助餐饮食 + TTI（25 mg/kg）；（3）标准饮食。TTI减少了患有MetS的动物的食物摄入量。然而，各研究组之间的体重增加没有差异。使用TTI后血脂异常参数没有差异，只有接受标准饮食的组显示极低密度脂蛋白（VLDL）和甘油三酯（TG）较低（Kruskal-Wallis检验，P < 0.05）。各组之间白细胞介素-6（IL-6）的产生没有差异。有趣的是，接受TTI的动物体内肿瘤坏死因子-α（TNF-α）较低。我们的结果证实了TTI在MetS模型中的促饱腹感作用。此外，我们表明添加到自助餐饮食中的TTI可能会减轻炎症，而与体重减轻无关。这使TTI成为研究其作为MetS治疗辅助剂有效性的候选物。
