Nutrition and Obesity Group, Department of Nutrition and Food Science, University of the Basque Country, Vitoria, Spain; CIBERobn Physiopathology of Obesity and Nutrition, Institute of Health Carlos III, Madrid, Spain.
Nutrition. 2013 Nov-Dec;29(11-12):1374-80. doi: 10.1016/j.nut.2013.04.014. Epub 2013 Sep 5.
The aim of this study was to examine whether resveratrol might represent a promising therapeutic tool with which to combat adipose tissue chronic inflammation in a model of genetic obesity and to link its anti-inflammatory activity with its effect on body fat reduction.
Twenty 6-wk-old male Zucker (fa/fa) rats were randomly distributed into two experimental groups. Resveratrol (RSV) was given orally (15 mg/kg body weight/d in RSV group) by means of an orogastric catheter for 6 wk. Enzyme activities were measured spectrophotometrically or fluorimetrically. Gene and protein expressions were analyzed by reverse transcriptase polymerase chain reaction and Western blot respectively. Cytokine concentrations and the activity of nuclear factor κ-light-chain-enhancer of activated β cells (NF-κB) were measured by using commercial kits.
RSV reduced the weight of internal adipose tissues. In epididymal depot glucose-6P-dehydrogenase, acetyl-CoA carboxylase activities, as well as lipoprotein lipase expression and activity were reduced by RSV. The expression of hormone-sensitive lipase was increased, and that of the cluster of differentiation 36 was reduced. Serum concentrations of tumor necrosis factor-α, monocyte chemoattractant protein 1, and C-reactive protein were lower in the RSV-treated group than in the control group. Protein expression of interleukin-6 and the activity of NF-κB, were decreased by RSV.
The present results provide evidence that fatty acid uptake and lipolysis are metabolic pathways involved in the response of adipose tissue to RSV. This polyphenol modulates plasma cytokine levels partially by reducing macrophage infiltration in adipose tissue and inhibiting NF-κB activity.
本研究旨在探讨白藜芦醇是否可能成为一种有前途的治疗工具，用于对抗遗传肥胖模型中脂肪组织的慢性炎症，并将其抗炎活性与其减少体脂肪的作用联系起来。
20 只 6 周龄雄性 Zucker（fa/fa）大鼠被随机分为两组。白藜芦醇（RSV）通过口腔胃管以 15mg/kg 体重/天的剂量（RSV 组）口服给予 6 周。酶活性通过分光光度法或荧光法进行测量。基因和蛋白质表达分别通过逆转录聚合酶链反应和 Western blot 进行分析。细胞因子浓度和核因子κ-轻链增强子活化β细胞（NF-κB）的活性通过商业试剂盒进行测量。
RSV 降低了内部脂肪组织的重量。在附睾脂肪组织中，葡萄糖-6-磷酸脱氢酶、乙酰辅酶 A 羧化酶的活性以及脂蛋白脂肪酶的表达和活性均被 RSV 降低。激素敏感脂肪酶的表达增加，而分化群 36 的表达减少。与对照组相比，RSV 治疗组血清肿瘤坏死因子-α、单核细胞趋化蛋白 1 和 C 反应蛋白的浓度较低。白藜芦醇还降低了白细胞介素 6 的蛋白表达和 NF-κB 的活性。
本研究结果提供了证据表明，脂肪酸摄取和脂肪分解是脂肪组织对 RSV 反应的代谢途径。这种多酚通过减少脂肪组织中巨噬细胞浸润并抑制 NF-κB 活性，部分调节血浆细胞因子水平。
