Castagnola Anaïs, Mulley Geraldine, Davis Nathaniel, Waterfield Nicholas, Stock S Patricia
Center for Insect Science, University of Arizona, Tucson, AZ 85721, USA.
School of Biological Sciences, University of Reading, Whiteknights, Reading RG6 6AJ, UK.
Toxins (Basel). 2016 Sep 29;8(10):287. doi: 10.3390/toxins8100287.
In this study, we assessed pirAB toxin transcription in (strain TT01) (Enterobacteriaceae) by comparing mRNA abundance under in vivo and in vitro conditions. In vivo assays considered both natural and forced infections with two lepidopteran hosts: and . Three portals of entry were utilized for the forced infection assays: (a) integument; (b) the digestive route (via mouth and anus); and (c) the tracheal route (via spiracles). We also assessed transcription during the course of a natural infection; this is when the bacteria are delivered by nematodes. Transcript abundance in was higher than in at two of the observed time points: 15 and 18 h. Expression of reached above endogenous control levels at 22 h in but not in . Overall, transcripts were not as highly expressed in as in from 15 to 22 h. This is the first study to directly compare toxin transcript production considering different portals of entry.
在本研究中，我们通过比较体内和体外条件下的mRNA丰度，评估了（菌株TT01）（肠杆菌科）中pirAB毒素的转录情况。体内试验考虑了两种鳞翅目宿主的自然感染和强制感染： 和 。强制感染试验采用了三个感染途径：（a）体表；（b）消化途径（经口和肛门）；以及（c）气管途径（经气门）。我们还评估了自然感染过程中的 转录情况；即在细菌由 线虫传播时的转录情况。在观察到的两个时间点（15小时和18小时）， 中的转录本丰度高于 。 在22小时时 的表达达到了内源性对照水平以上，但 在该时间点未达到。总体而言，在15至22小时内， 转录本在 中的表达不如在 中高。这是第一项直接比较考虑不同感染途径时 毒素转录本产生情况的研究。
