Department of Biomedical Engineering, Faculty of Engineering, Tel Aviv University, Ramat Aviv, Tel Aviv, Israel.
Department of Oral Biology, School of Dental Medicine, Tel Aviv University, Ramat Aviv, Tel Aviv, Israel.
Magn Reson Med. 2017 Sep;78(3):860-870. doi: 10.1002/mrm.26472. Epub 2016 Sep 30.
Osteoporosis is characterized by a decrease in bone mineral density (BMD). A preliminary stage of the disease is progressive bone marrow adiposity, caused by imbalance between osteogenesis and adipogenesis in the marrow. Detection of osteoporosis relies on the quantification of BMD with techniques such as dual-energy X-ray absorptiometry. This work aimed to detect bone marrow changes in an experimental model of osteopenia using a low-field tabletop NMR scanner.
An experiment was performed on 32 female rats, 3 months old, 16 of which were ovariectomized (OVX) and 16 were sham-operated (sham). The femur and tibia from both hind limbs were isolated and underwent ex vivo NMR scans at four time points after the OVX and sham operations. NMR scans were complemented by BMD measurements and histology.
Significant changes in the bone marrow of ovariectomized rats, relative to sham operated rats, were observed after 3.5 and 4.5 months. Bone marrow adiposity was detected by significant changes in T and T relaxation times, and in the diffusion coefficient.
This study suggests a potential detection of changes to the bone marrow using a tabletop NMR device. Clinical translation may facilitate screening, early detection of bone weakening as a result of estrogen withdrawal, and monitoring of treatment efficacy. Magn Reson Med 78:860-870, 2017. © 2016 International Society for Magnetic Resonance in Medicine.
骨质疏松症的特征是骨密度（BMD）下降。该疾病的早期阶段是骨髓脂肪增多，这是由骨髓中成骨和脂肪生成之间的失衡引起的。骨质疏松症的检测依赖于双能 X 射线吸收法等技术对 BMD 的定量。本研究旨在使用低场台式 NMR 扫描仪检测骨质疏松症实验模型中的骨髓变化。
对 32 只 3 个月大的雌性大鼠进行了实验，其中 16 只接受了卵巢切除术（OVX），16 只接受了假手术（sham）。对双侧后肢的股骨和胫骨进行分离，并在 OVX 和 sham 手术后的四个时间点进行离体 NMR 扫描。NMR 扫描与 BMD 测量和组织学相结合。
与 sham 手术组相比，OVX 手术后 3.5 和 4.5 个月时，OVX 大鼠的骨髓发生了明显变化。骨髓脂肪增多可通过 T 和 T 弛豫时间以及扩散系数的显著变化来检测。
本研究表明，使用台式 NMR 设备有可能检测骨髓的变化。临床转化可能有助于筛选、早期发现雌激素缺乏引起的骨弱化以及监测治疗效果。磁共振医学 78:860-870,2017。© 2016 国际磁共振学会。
