Zhang Long, Chen Xiao-Long, Hu Qiang, Ruan Zhe-Pu, Chen Kai, Li Shun-Peng, Jiang Jian-Dong
Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, 210095 Nanjing, PR China.
Int J Syst Evol Microbiol. 2016 Dec;66(12):5399-5405. doi: 10.1099/ijsem.0.001531. Epub 2016 Sep 29.
A novel Gram-stain-positive, non-motile, rod-shaped bacterial strain designated LIP-1T was isolated from the contaminated soil of a pesticide factory in Xinyi, China, was investigated for its taxonomic allocation by a polyphasic approach. Cell growth occurred at 16-37 °C (optimum, 30 °C), in the presence of 0-2.0 % (w/v) NaCl (optimum, 0 %) and at pH 6.0-9.0 (optimum, pH 7.0). The major fatty acids of strain LIP-1T were anteiso-C15 : 0 (50.8 %), iso-C16 : 0 (17.6 %) and anteiso-C17 : 0 (17.4 %). The cell-wall peptidoglycan type was B2δ with 2,4-diaminobutyric acid as the diagnostic diamino acid. The major polar lipids were diphosphatidylglycerol and two unidentified glycolipids. The major menaquinones were MK-12 and MK-11. The genomic DNA G+C content was approximately 63.8 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain LIP-1T formed a distinct clade within the radiation of the family Microbacteriaceae and had the highest sequence similarity with Microbacterium ginsengisoli Gsoil 259T (96.01 %) followed by Cryobacterium arcticum SK1T (94.94 %). On the basis of the phylogenetic analyses and distinct phenotypic characteristics, a new genus, namely Huakuichenia gen. nov., is proposed, harbouring the novel species Huakuichenia soli gen. nov., sp. nov. with the type strain LIP-1T (=CCTCC AB 2015422T=KCTC 39698T).
从中国新沂一家农药厂受污染土壤中分离出一株新的革兰氏染色阳性、无运动性、杆状细菌菌株，命名为LIP-1T，采用多相分类法对其进行分类地位研究。该菌株在16-37 °C（最适温度30 °C）、0-2.0 %（w/v）NaCl（最适浓度0 %）和pH 6.0-9.0（最适pH 7.0）条件下生长。菌株LIP-1T的主要脂肪酸为anteiso-C15 : 0（50.8 %）、iso-C16 : 0（17.6 %）和anteiso-C17 : 0（17.4 %）。细胞壁肽聚糖类型为B2δ，以2,4-二氨基丁酸作为诊断性二氨基酸。主要极性脂为双磷脂酰甘油和两种未鉴定的糖脂。主要甲基萘醌为MK-12和MK-11。基因组DNA G+C含量约为63.8 mol%。基于16S rRNA基因序列的系统发育分析表明，菌株LIP-1T在微小杆菌科的辐射范围内形成一个独特的分支，与参地微小杆菌Gsoil 259T（96.01 %）序列相似性最高，其次是北极嗜冷杆菌SK1T（94.94 %）。基于系统发育分析和独特的表型特征，提出一个新属，即华癸菌属（Huakuichenia gen. nov.），包含新种土壤华癸菌（Huakuichenia soli gen. nov., sp. nov.），模式菌株为LIP-1T（=CCTCC AB 2015422T=KCTC 39698T）。
