Agricultural Microbiology Division, National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2013 Nov;63(Pt 11):4052-4057. doi: 10.1099/ijs.0.049817-0. Epub 2013 May 31.
A novel Gram-stain-positive, non-motile, rod-shaped bacterium, designated 5GHs34-4(T), was isolated from greenhouse soil in Yongin, Republic of Korea. Growth occurred in the temperature range of 10-37 °C (optimum 28-30 °C) and at pH 5.0-9.0 (optimum pH 7.0). It can tolerate up to 3 % (w/v) NaCl. The strain showed 16S rRNA gene sequence similarity levels of 95.1-97.0 % with species of the genus Leifsonia, 95.7-96.7 % with species of the genus Herbiconiux, 95.1-96.4 % with species of the genus Salinibacterium and 96.1 % with Labedella gwakjiensis and Homoserinimonas aerilata. The highest sequence similarities (97.0 %) were with Leifsonia aquatica JCM 1368(T), Leifsonia poae VKM Ac-1401(T) and Leifsonia psychrotolerans LI1(T). The peptidoglycan type determined for strain 5GHs34-4(T) was B2γ with dl-2,4-diaminobutyric acid at position 3. The murein was of the acetyl type. The polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol and two unknown glycolipids. The menaquinones detected were MK-13, MK-12 and MK-14, and the major fatty acids were summed feature 8 (C18 : 1ω7c and/or C18 : 1ω6c), anteiso-C17 : 0 and anteiso-C15 : 0. The phenotypic and phylogenetic traits of strain 5GHs34-4(T) differed in some respects from those of members of the family Microbacteriaceae. Therefore, strain 5GHs34-4(T) is considered to represent a novel species of a new genus in the family Microbacteriaceae, for which the name Rudaibacter terrae gen. nov., sp. nov. is proposed. The type strain is 5GHs34-4(T) ( = KACC 15523(T) = NBRC 108754(T)).
一株新型革兰氏阳性、非运动、杆状细菌，被命名为 5GHs34-4(T)，从韩国龙仁市温室土壤中分离得到。生长温度范围为 10-37°C（最佳温度为 28-30°C），pH 值范围为 5.0-9.0（最佳 pH 值为 7.0）。它可以耐受高达 3%（w/v）的 NaCl。该菌株与利夫森氏菌属的种、与 Herbiconiux 属的种、与 Salinibacterium 属的种和与 Labedella gwakjiensis 和 Homoserinimonas aerilata 的 16S rRNA 基因序列相似性水平分别为 95.1-97.0%、95.7-96.7%、95.1-96.4%和 96.1%。与 Leifsonia aquatica JCM 1368(T)、Leifsonia poae VKM Ac-1401(T) 和 Leifsonia psychrotolerans LI1(T) 的最高序列相似性（97.0%）。菌株 5GHs34-4(T) 的肽聚糖类型为 B2γ，具有 dl-2,4-二氨基丁酸位于位置 3。肽聚糖为乙酰型。极性脂类由双磷脂酰甘油、磷脂酰甘油和两种未知的糖脂组成。检测到的menaquinones 为 MK-13、MK-12 和 MK-14，主要脂肪酸为 summed feature 8（C18:1ω7c 和/或 C18:1ω6c）、anteiso-C17:0 和 anteiso-C15:0。菌株 5GHs34-4(T) 的表型和系统发育特征在某些方面与微杆菌科成员不同。因此，菌株 5GHs34-4(T) 被认为代表了微杆菌科一个新属的一个新种，建议将其命名为 Rudaibacter terrae gen. nov.，sp. nov.。模式菌株为 5GHs34-4(T)（=KACC 15523(T)=NBRC 108754(T)）。
