Korean Agricultural Culture Collection (KACC), National Academy of Agricultural Science, Rural Development Administration, Suwon 441-707, Republic of Korea.
NITE Biological Resource Center (NBRC), National Institute of Technology and Evaluation, 2-5-8 Kazusakamatari, Kisarazu, Chiba 292-0818, Japan.
Int J Syst Evol Microbiol. 2013 Apr;63(Pt 4):1403-1410. doi: 10.1099/ijs.0.042945-0. Epub 2012 Jul 20.
A Gram-stain-positive, non-motile rod, designated strain SGM3-12(T), was isolated from paddy soil in Suwon, Republic of Korea. 16S rRNA gene sequence analysis revealed that the strain represented a novel member of the family Microbacteriaceae. The nearest phylogenetic neighbour was Leifsonia kribbensis MSL-13(T) (97.4 % 16S rRNA gene sequence similarity). Strain SGM3-12(T) and Leifsonia kribbensis MSL-13(T) formed a distinct cluster within the family Microbacteriaceae. Strain SGM3-12(T) contained MK-12(H2) and MK-11(H2) as the predominant menaquinones with moderate amounts of MK-12 and MK-11; anteiso-C15 : 0 and iso-C16 : 0 as the major cellular fatty acids (>10 % of total); and diphosphatidylglycerol, phosphatidylglycerol and unidentified glycolipids as the polar lipids. The peptidoglycan type of the isolate was B1δ with L-Lys as the diagnostic cell-wall diamino acid. On the basis of these results, strain SGM3-12(T) represents a novel species within a new genus, for which the name Lysinimonas soli gen. nov., sp. nov. is proposed (the type strain of the type species is SGM3-12(T) = KACC 13362(T) = NBRC 107106(T)). It is also proposed that Leifsonia kribbensis be transferred to this genus as Lysinimonas kribbensis comb. nov. (the type strain is MSL-13(T) = DSM 19272(T) = JCM 16015(T) = KACC 21108(T) = KCTC 19267(T)).
一株革兰氏阳性、非运动的杆状菌，被命名为 SGM3-12(T) 菌株，从韩国水原的稻田土壤中分离得到。16S rRNA 基因序列分析表明，该菌株代表微杆菌科的一个新成员。其最接近的系统发育近邻是 Leifsonia kribbensis MSL-13(T)（97.4% 16S rRNA 基因序列相似性）。SGM3-12(T) 菌株和 Leifsonia kribbensis MSL-13(T) 在微杆菌科内形成了一个独特的聚类。SGM3-12(T) 菌株含有 MK-12(H2) 和 MK-11(H2) 作为主要的menaquinones，并有中等数量的 MK-12 和 MK-11；anteiso-C15:0 和 iso-C16:0 作为主要的细胞脂肪酸（>10%总脂肪酸）；二磷脂酰甘油、磷脂酰甘油和未鉴定的糖脂作为极性脂。该分离株的肽聚糖类型为 B1δ，其特征性的细胞壁二氨基庚二酸为 L-Lys。基于这些结果，SGM3-12(T) 菌株代表了一个新属中的一个新种，建议将其命名为 Lysinimonas soli 属。nov.，sp. nov.（模式种为 SGM3-12(T) = KACC 13362(T) = NBRC 107106(T)）。还建议将 Leifsonia kribbensis 转移到该属中，作为 Lysinimonas kribbensis comb. nov.（模式株为 MSL-13(T) = DSM 19272(T) = JCM 16015(T) = KACC 21108(T) = KCTC 19267(T)）。
