Walaas S I, Wang J K, Albert K A, Greengard P
Laboratory of Molecular and Cellular Neuroscience, Rockefeller University, New York, New York.
J Neurochem. 1989 Oct;53(4):1199-202. doi: 10.1111/j.1471-4159.1989.tb07415.x.
When brain tissue is subjected to subcellular fractionation, both calcium/diacylglycerol-dependent protein kinase (protein kinase C) and an 87-kilodalton (kDa) protein substrate for this enzyme are enriched in the crude nerve terminal fraction. The present study, using chemical and surgical lesions of neurons in the rat neostriatum and substantia nigra, has examined whether the 87-kDa protein is colocalized with protein kinase C in identified neurons and nerve terminals. Our results show that, in the basal ganglia, protein kinase C is highly enriched in local striatal neurons and the striatonigral fibers and terminals. In contrast, the 87-kDa protein appears to be widely and evenly distributed in both neuronal and nonneuronal cells. The 87-kDa protein may therefore mediate functions of protein kinase C not restricted to nerve terminals.
当脑组织进行亚细胞分级分离时，钙/二酰基甘油依赖性蛋白激酶（蛋白激酶C）及其该酶的一种87千道尔顿（kDa）蛋白底物在粗制神经终末分级分离物中均得到富集。本研究利用大鼠新纹状体和黑质神经元的化学性和手术性损伤，检测了该87-kDa蛋白是否与蛋白激酶C在已鉴定的神经元和神经终末中共定位。我们的结果显示，在基底神经节中，蛋白激酶C在局部纹状体神经元以及纹状体黑质纤维和终末中高度富集。相比之下，该87-kDa蛋白似乎广泛且均匀地分布于神经元和非神经元细胞中。因此，该87-kDa蛋白可能介导蛋白激酶C不限于神经终末的功能。
