健康女性和诊断为细菌性阴道病的女性阴道加德纳菌的药敏性及阴道溶素

Antimicrobial susceptibility and vaginolysin in Gardnerella vaginalis from healthy and bacterial vaginosis diagnosed women.

作者信息

Knupp de Souza Daniele Maria, Diniz Claudio Galuppo, Filho Didier Silveira Castellano, Andrade de Oliveira Laura Maria, Coelho Débora Martins, Talha Luciana De Souza, Nascimento Thiago César do, Ferreira-Machado Alessandra Barbosa, Silva Vânia Lúcia da

机构信息

Federal University of Juiz de Fora, Juiz de Fora, Minas Gerais, Brazil.

出版信息

J Infect Dev Ctries. 2016 Sep 30;10(9):913-919. doi: 10.3855/jidc.7161.

DOI:10.3855/jidc.7161

PMID:27694723

Abstract

INTRODUCTION

Bacterial vaginosis (BV) is a syndrome related to Gardnerella vaginalis and is characterized by an imbalance in the vaginal microbiota. This work focused on the evaluation of antimicrobial susceptibility patterns and the occurrence of the vaginolysin (vly) gene in G. vaginalis isolated from BV and non-BV patients.

METHODOLOGY

The vaginal secretions were collected randomly and processed for G. vaginalis isolation. The isolates were presumptively identified by β-hemolysis and oxidase and catalase tests. Polymerase chain reaction (PCR) was performed to confirm bacterial identity and to detect the vly gene. Antimicrobial susceptibility patterns were determined.

RESULTS

Of 89 patients, G. vaginalis was isolated from 42 (37 BV and 5 non-BV), and 204 isolates were selected (179 from BV and 25 non-BV). The vly gene was detected in all G. vaginalis isolated from non-BV women and in 98.3% of the bacteria from BV patients. High resistance was observed for ampicillin (54.4%), metronidazole (59.8%), tinidazole (60.3%) and secnidazole (71.6%).

CONCLUSIONS

Further studies are needed to better address the role of G. vaginalis and the vly gene in BV pathogenesis.

摘要

引言

细菌性阴道病（BV）是一种与阴道加德纳菌相关的综合征，其特征是阴道微生物群失衡。这项工作重点评估了从BV患者和非BV患者中分离出的阴道加德纳菌的抗菌药敏模式以及阴道溶素（vly）基因的出现情况。

方法

随机收集阴道分泌物并进行处理以分离阴道加德纳菌。通过β-溶血、氧化酶和过氧化氢酶试验对分离株进行初步鉴定。进行聚合酶链反应（PCR）以确认细菌身份并检测vly基因。确定抗菌药敏模式。

结果

在89名患者中，从42名患者中分离出阴道加德纳菌（37名BV患者和5名非BV患者），共选择了204株分离株（179株来自BV患者，25株来自非BV患者）。在从非BV女性中分离出的所有阴道加德纳菌以及98.3%的BV患者细菌中检测到vly基因。观察到对氨苄西林（54.4%）、甲硝唑（59.8%）、替硝唑（60.3%）和塞克硝唑（71.6%）的高耐药性。