Ackermann Hans-W
Department of Microbiology-Immunology and Infectiology, Medical School, Laval University, Quebec, QC G1K 7P4, Canada.
Microorganisms. 2013 Dec 24;2(1):1-10. doi: 10.3390/microorganisms2010001.
Two hundred and sixty publications from 2007 to 2012 were classified according to the quality of electron micrographs; namely as good (71); mediocre (21); or poor (168). Publications were from 37 countries; appeared in 77 journals; and included micrographs produced with about 60 models of electron microscopes. The quality of the micrographs was not linked to any country; journal; or electron microscope. Main problems were poor contrast; positive staining; low magnification; and small image size. Unsharp images were frequent. Many phage descriptions were silent on virus purification; magnification control; even the type of electron microscope and stain used. The deterioration in phage electron microscopy can be attributed to the absence of working instructions and electron microscopy courses; incompetent authors and reviewers; and lenient journals. All these factors are able to cause a gradual lowering of standards.
2007年至2012年期间的260篇出版物根据电子显微镜照片的质量进行了分类，即分为优质（71篇）、中等（21篇）或劣质（168篇）。这些出版物来自37个国家，发表在77种期刊上，包括使用约60种型号电子显微镜制作的显微照片。显微照片的质量与任何国家、期刊或电子显微镜均无关联。主要问题包括对比度差、阳性染色、放大倍数低和图像尺寸小。图像不清晰的情况很常见。许多噬菌体描述未提及病毒纯化、放大倍数控制，甚至未提及所使用的电子显微镜类型和染色方法。噬菌体电子显微镜技术的退化可归因于缺乏操作指南和电子显微镜课程、作者和审稿人能力不足以及期刊把关不严。所有这些因素都可能导致标准逐渐降低。
